caa a22 4500 475737938 CHVBK 20180406123452.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s004300000042 doi (NATIONALLICENCE)springer-10.1007/s004300000042 Inducing protective antibodies against ring-infected erythrocyte surface peptide antigen of Plasmodium falciparum using immunostimulating complex (ISCOMs) delivery [Elektronische Daten] [Neeraj Chopra, Shukla Biswas, Beena Thomas, Leenu Sabhnani, D. N. Rao] In the present study, synthetic peptides (EENVEHDA)2 [(oc)2] and (DDEHVEEPTVA)2 [(un)2] of ring-infected erythrocyte surface antigen (RESA) of Plasmodium falciparum were linked with palmitic acid and entrapped in immunostimulating complexes (ISCOMs). The immunogenicity of the peptide(s) and mixture of peptides were studied in mice with different genetic background. Peptide(s) entrapped in ISCOMs using a low-dose immunization strategy generated high-titer as well as high-affinity antibodies. Interestingly, no genetic restriction of the immune response was observed in any of the strains studied. The IgG subclass pattern with the peptide(s) showed predominately IgG2a/2b isotypes, while with the mixed peptide formulation, (un)2-specific IgG isotype pattern showed induction of both IgG1 and IgG2a/2b isotypes. These cytophilic antibodies inhibited the ring as well as schizont stage and total parasite growth during in vitro merozoite reinvasion inhibition study. In the mixed peptide preparation, the same pattern of immune response was achieved as that of individual peptide(s) using ISCOMs delivery. Therefore, the entrapment of otherwise poorly immunogenic synthetic peptides in ISCOMs resulted in increased immunogenicity followed by strong secondary response and can be adopted for developing subunit immunogen formulation against malarial parasite. Springer-Verlag Berlin Heidelberg, 2000 Key words Ring-infected erythrocyte surface antigen peptides nationallicence Immunostimulating complexes nationallicence Inbred mice nationallicence Protective antibodies nationallicence Chopra Neeraj Department of Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New Delhi-110 029, India e-mail: dnrao@medinst.ernet.in Tel.: +91-11-6593545; Fax: +91-11-6862663, IN aut Biswas Shukla Malaria Research Centre, 22 Sham Nath Marg, Delhi-110 054, India, IN aut Thomas Beena Department of Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New Delhi-110 029, India e-mail: dnrao@medinst.ernet.in Tel.: +91-11-6593545; Fax: +91-11-6862663, IN aut Sabhnani Leenu Department of Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New Delhi-110 029, India e-mail: dnrao@medinst.ernet.in Tel.: +91-11-6593545; Fax: +91-11-6862663, IN aut Rao D. N. Department of Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New Delhi-110 029, India e-mail: dnrao@medinst.ernet.in Tel.: +91-11-6593545; Fax: +91-11-6862663, IN aut https://doi.org/10.1007/s004300000042 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004300000042 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chopra Neeraj Department of Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New Delhi-110 029, India e-mail: dnrao@medinst.ernet.in Tel.: +91-11-6593545; Fax: +91-11-6862663, IN aut NATIONALLICENCE 700 1- Biswas Shukla Malaria Research Centre, 22 Sham Nath Marg, Delhi-110 054, India, IN aut NATIONALLICENCE 700 1- Thomas Beena Department of Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New Delhi-110 029, India e-mail: dnrao@medinst.ernet.in Tel.: +91-11-6593545; Fax: +91-11-6862663, IN aut NATIONALLICENCE 700 1- Sabhnani Leenu Department of Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New Delhi-110 029, India e-mail: dnrao@medinst.ernet.in Tel.: +91-11-6593545; Fax: +91-11-6862663, IN aut NATIONALLICENCE 700 1- Rao D. N. Department of Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New Delhi-110 029, India e-mail: dnrao@medinst.ernet.in Tel.: +91-11-6593545; Fax: +91-11-6862663, IN aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer