caa a22 4500 475737970 CHVBK 20180406123452.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/PL00008258 doi (NATIONALLICENCE)springer-10.1007/PL00008258 IL-5 is essential for vaccine-induced protection and for resolution of primary infection in murine filariasis [Elektronische Daten] [Coralie Martin, Khaled M. Al-Qaoud, Marie-Noëlle Ungeheuer, Kerstin Paehle, Phat N. Vuong, Odile Bain, Bernhard Fleischer, Achim Hoerauf] The pathways conferring immunity to human filariases are not well known, in part because human-pathogenic filariae do not complete a full life cycle in laboratory mice. We have used the only fully permissive infection of mice with filariae, i.e., infection of BALB/c mice with the rodent filarial nematode Litomosoides sigmodontis. Our previous results showed that worm development is inversely correlated with Th2 cytokine production and eosinophilia. The scope of the present study was to directly elucidate the role of interleukin-5 (IL-5) and eosinophils in controlling the development of L. sigmodontis after vaccination and in primary infection. BALB/c mice immunized with irradiated third-stage larvae (L3) were confirmed to have elevated IL-5 levels as well as high subcutaneous eosinophilia and to attack and reduce incoming larvae within the first 2 days, resulting in 70% reduction of worm load. Treatment of vaccinated mice with anti-IL-5 antibody (TRFK-5) suppressed both blood and tissue eosinophilia and completely abolished protection. This demonstrates, for the first time in a fully permissive filarial infection, that IL-5 is essential for protection induced by irradiated L3 larvae. In contrast, in primary-infected mice, anti-IL-5 treatment did not modify filarial infection within the 1st month, most likely because during primary infection IL-5-dependent mechanisms such as subcutaneous eosinophilia are induced too late to disturb worm establishment. However, there is a role for IL-5 late in primary infection where neutrophil-dependent worm encapsulation is also under the control of IL-5. Springer-Verlag Berlin Heidelberg, 2000 Key words Interleukin-5 nationallicence Murine filariasis nationallicence Eosinophils nationallicence Vaccine-induced protection nationallicence Helminth infection nationallicence Martin Coralie Institut de Systématique CNRS FR 1541, Biologie Parasitaire, Muséum National d'Histoire Naturelle, 61 rue Buffon, 75231 Paris Cedex 05, France e-mail: bain@mnhn.fr Tel.: +33-1-40793497; Fax: +33-1-40793499, FR aut Al-Qaoud Khaled M. Bernhard-Nocht-Institute of Tropical Medicine, 20359 Hamburg, Germany, DE aut Ungeheuer Marie-Noëlle Institut de Systématique CNRS FR 1541, Biologie Parasitaire, Muséum National d'Histoire Naturelle, 61 rue Buffon, 75231 Paris Cedex 05, France e-mail: bain@mnhn.fr Tel.: +33-1-40793497; Fax: +33-1-40793499, FR aut Paehle Kerstin Bernhard-Nocht-Institute of Tropical Medicine, 20359 Hamburg, Germany, DE aut Vuong Phat N. Laboratoire de Cytologie et Anatomopathologie, Hôpital St Michel, Paris, France, FR aut Bain Odile Institut de Systématique CNRS FR 1541, Biologie Parasitaire, Muséum National d'Histoire Naturelle, 61 rue Buffon, 75231 Paris Cedex 05, France e-mail: bain@mnhn.fr Tel.: +33-1-40793497; Fax: +33-1-40793499, FR aut Fleischer Bernhard Bernhard-Nocht-Institute of Tropical Medicine, 20359 Hamburg, Germany, DE aut Hoerauf Achim Bernhard-Nocht-Institute of Tropical Medicine, 20359 Hamburg, Germany, DE aut https://doi.org/10.1007/PL00008258 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00008258 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Martin Coralie Institut de Systématique CNRS FR 1541, Biologie Parasitaire, Muséum National d'Histoire Naturelle, 61 rue Buffon, 75231 Paris Cedex 05, France e-mail: bain@mnhn.fr Tel.: +33-1-40793497; Fax: +33-1-40793499, FR aut NATIONALLICENCE 700 1- Al-Qaoud Khaled M. Bernhard-Nocht-Institute of Tropical Medicine, 20359 Hamburg, Germany, DE aut NATIONALLICENCE 700 1- Ungeheuer Marie-Noëlle Institut de Systématique CNRS FR 1541, Biologie Parasitaire, Muséum National d'Histoire Naturelle, 61 rue Buffon, 75231 Paris Cedex 05, France e-mail: bain@mnhn.fr Tel.: +33-1-40793497; Fax: +33-1-40793499, FR aut NATIONALLICENCE 700 1- Paehle Kerstin Bernhard-Nocht-Institute of Tropical Medicine, 20359 Hamburg, Germany, DE aut NATIONALLICENCE 700 1- Vuong Phat N. Laboratoire de Cytologie et Anatomopathologie, Hôpital St Michel, Paris, France, FR aut NATIONALLICENCE 700 1- Bain Odile Institut de Systématique CNRS FR 1541, Biologie Parasitaire, Muséum National d'Histoire Naturelle, 61 rue Buffon, 75231 Paris Cedex 05, France e-mail: bain@mnhn.fr Tel.: +33-1-40793497; Fax: +33-1-40793499, FR aut NATIONALLICENCE 700 1- Fleischer Bernhard Bernhard-Nocht-Institute of Tropical Medicine, 20359 Hamburg, Germany, DE aut NATIONALLICENCE 700 1- Hoerauf Achim Bernhard-Nocht-Institute of Tropical Medicine, 20359 Hamburg, Germany, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer