caa a22 4500 475742362 CHVBK 20180406123503.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s002400050001 doi (NATIONALLICENCE)springer-10.1007/s002400050001 Interstitial fluid pressure is increased in renal cell carcinoma xenografts [Elektronische Daten] [Detlef Rohde, Christoph Wiesner, Dirk Graf, Johannes Wolff, Laszlo Füzesi, Gerhard Jakse] The purpose of this study was to test the hypothesis that renal cell carcinoma (RCC) exhibits an increased intratumoral interstitial fluid pressure (IT-IFP). Therefore, resected tumors from human primary (n = 23) or metastatic RCC (n = 3) were xenografted in SCID mice. The IFP of single tumor nodules (n = 65) and normal mouse tissue (n = 195) was measured by means of the "wick-in-needle” technique. Data demonstrate that the mean IT-IFP at neoplasia was 35 times greater than in normal tissue, and decreased precipitously at the tumor boundary. IT-IFP values tended to increase with the grade of malignancy of the tumor cells and tumor size. The mean IT-IFP of xenografts derived from primary RCC was twice as high as that from metastatic RCC tissue. These findings indicate a biophysical barrier to drug delivery in RCC; this may, in concert with cellular-based drug resistance mechanisms, be an additional explanation for resistance of the tumor to certain blood-borne anticancer therapies. Springer-Verlag Berlin Heidelberg, 2000 Key words Drug delivery system nationallicence Mice, nude nationallicence Neoplasm, metastasis, physiopathology nationallicence Pressure nationallicence Neoplasm, kidney nationallicence Rohde Detlef Department of Urology, Medical Faculty, University of Aachen, Pauwelsstrasse 30, 52057 Aachen, Germany, DE aut Wiesner Christoph Department of Urology, Medical Faculty, University of Aachen, Pauwelsstrasse 30, 52057 Aachen, Germany, DE aut Graf Dirk Department of Urology, Medical Faculty, University of Aachen, Pauwelsstrasse 30, 52057 Aachen, Germany, DE aut Wolff Johannes Department of Urology, Medical Faculty, University of Aachen, Pauwelsstrasse 30, 52057 Aachen, Germany, DE aut Füzesi Laszlo Department of Pathology, Medical Faculty, University of Aachen, Pauwelsstrasse 30, 52057 Aachen, Germany, DE aut Jakse Gerhard Department of Urology, Medical Faculty, University of Aachen, Pauwelsstrasse 30, 52057 Aachen, Germany, DE aut https://doi.org/10.1007/s002400050001 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002400050001 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rohde Detlef Department of Urology, Medical Faculty, University of Aachen, Pauwelsstrasse 30, 52057 Aachen, Germany, DE aut NATIONALLICENCE 700 1- Wiesner Christoph Department of Urology, Medical Faculty, University of Aachen, Pauwelsstrasse 30, 52057 Aachen, Germany, DE aut NATIONALLICENCE 700 1- Graf Dirk Department of Urology, Medical Faculty, University of Aachen, Pauwelsstrasse 30, 52057 Aachen, Germany, DE aut NATIONALLICENCE 700 1- Wolff Johannes Department of Urology, Medical Faculty, University of Aachen, Pauwelsstrasse 30, 52057 Aachen, Germany, DE aut NATIONALLICENCE 700 1- Füzesi Laszlo Department of Pathology, Medical Faculty, University of Aachen, Pauwelsstrasse 30, 52057 Aachen, Germany, DE aut NATIONALLICENCE 700 1- Jakse Gerhard Department of Urology, Medical Faculty, University of Aachen, Pauwelsstrasse 30, 52057 Aachen, Germany, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer