caa a22 4500 475742389 CHVBK 20180406123503.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s002400050012 doi (NATIONALLICENCE)springer-10.1007/s002400050012 Differential binding activities of lectins and neoglycoproteins in human testicular tumors [Elektronische Daten] [X.-C. Xu, U. Brinck, A. Schauer, H. -J. Gabius] Testicular germ-cell tumors, a morphologically and clinically diverse group of malignancies provide an ideal model for investigating the biology of glycoconjugates because the biosynthesis of oligosaccharide chains of glycoproteins monitored by plant/invertebrate lectins often changes during tumorigenesis, tumor progression, and metastasis. To investigate such changes in germ-cell tumors, we analyzed 67 surgical specimens from 31 seminomas, 32 embryonic carcinomas, and four choriocarcinomas using glyco- and immunohistochemistry that involved five plant/invertebrate lectins, 16 neoglycoproteins, and galectin-1 antibody. The results showed that some of these markers, such as melibiose-, lactose-, and β-N-acetylgalactosamine-BSA-biotin were clearly differentially expressed amongst these tumors and between primary and metastatic embryonic carcinomas. The differences in staining for positivity, intensity, and heterogeneity indicate that the differential display of glycoconjugates in tumor cells may be important in tumor growth, metastasis, or prognosis because subtypes of these tumors behave quite differently from one another. Furthermore, we also found identical staining for positivity between most neoglycoproteins and their corresponding lectins, though the staining intensity of neoglycoproteins was weaker. This suggests that neoglycoproteins may be useful markers to replace their plant lectins. Springer-Verlag Berlin Heidelberg, 2000 Key words Lectin nationallicence Glycoconjugates nationallicence Glycohistochemistry nationallicence Neoglycoprotein nationallicence Testicular tumors nationallicence Xu X.-C Department of Clinical Cancer Prevention, University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Blvd., Box 236, Houston, Texas 77030, USA e-mail: xxu@notes.mdacc.tmc.edu Tel.:+1-713-745-2940; Fax: +1-713-792-0628, US aut Brinck U. Institute of Pathology, Georg-August University, Göttingen, Germany, DE aut Schauer A. Institute of Pathology, Georg-August University, Göttingen, Germany, DE aut Gabius H. -J Institute of Physiological Chemistry, Ludwig-Maximilians University, Munich, Germany, DE aut https://doi.org/10.1007/s002400050012 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002400050012 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu X.-C Department of Clinical Cancer Prevention, University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Blvd., Box 236, Houston, Texas 77030, USA e-mail: xxu@notes.mdacc.tmc.edu Tel.:+1-713-745-2940; Fax: +1-713-792-0628, US aut NATIONALLICENCE 700 1- Brinck U. Institute of Pathology, Georg-August University, Göttingen, Germany, DE aut NATIONALLICENCE 700 1- Schauer A. Institute of Pathology, Georg-August University, Göttingen, Germany, DE aut NATIONALLICENCE 700 1- Gabius H. -J Institute of Physiological Chemistry, Ludwig-Maximilians University, Munich, Germany, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer