caa a22 4500 475742400 CHVBK 20180406123503.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s002400050011 doi (NATIONALLICENCE)springer-10.1007/s002400050011 Chromosomal numerical aberrations of exfoliated cells in the urine detected by fluorescence in situ hybridization: clinical implication for the detection of bladder cancer [Elektronische Daten] [T. Inoue, Y. Nasu, T. Tsushima, Y. Miyaji, T. Murakami, H. Kumon] Numerical aberrations of chromosomes 7 and 9 of exfoliated cells from the urinary tract were examined using fluorescence in situ hybridization (FISH). To minimize contamination with inflammatory, squamous and normal transitional cells all samples were stained using Giemsa's solution and clusters of transitional cells were selected for FISH analysis. Samples collected from 21 patients who had cystoscopic monitoring for bladder cancer were used in this study. Cystoscopy was positive in seven patients and five biopsy-proven transitional cell carcinomas were identified. Among the patients with cancer recurrence, numerical chromosomal aberrations were detected in four cases (sensitivity: 80%) and positive cytology in two cases. This preliminary pilot study shows FISH can be a useful tool for the detection of recurrence during a follow up of bladder cancer. Springer-Verlag Berlin Heidelberg, 2000 Key words Chromosomal numerical aberration nationallicence FISH nationallicence Exfoliated cell nationallicence Bladder cancer nationallicence Inoue T. Department of Urology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan e-mail: tsushima@med.okayama-u.ac.jp Tel.: +81 86 235 7285; Fax: +81 86 231 3986, JP aut Nasu Y. Department of Urology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan e-mail: tsushima@med.okayama-u.ac.jp Tel.: +81 86 235 7285; Fax: +81 86 231 3986, JP aut Tsushima T. Department of Urology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan e-mail: tsushima@med.okayama-u.ac.jp Tel.: +81 86 235 7285; Fax: +81 86 231 3986, JP aut Miyaji Y. Department of Urology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan e-mail: tsushima@med.okayama-u.ac.jp Tel.: +81 86 235 7285; Fax: +81 86 231 3986, JP aut Murakami T. Department of Urology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan e-mail: tsushima@med.okayama-u.ac.jp Tel.: +81 86 235 7285; Fax: +81 86 231 3986, JP aut Kumon H. Department of Urology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan e-mail: tsushima@med.okayama-u.ac.jp Tel.: +81 86 235 7285; Fax: +81 86 231 3986, JP aut https://doi.org/10.1007/s002400050011 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002400050011 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Inoue T. Department of Urology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan e-mail: tsushima@med.okayama-u.ac.jp Tel.: +81 86 235 7285; Fax: +81 86 231 3986, JP aut NATIONALLICENCE 700 1- Nasu Y. Department of Urology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan e-mail: tsushima@med.okayama-u.ac.jp Tel.: +81 86 235 7285; Fax: +81 86 231 3986, JP aut NATIONALLICENCE 700 1- Tsushima T. Department of Urology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan e-mail: tsushima@med.okayama-u.ac.jp Tel.: +81 86 235 7285; Fax: +81 86 231 3986, JP aut NATIONALLICENCE 700 1- Miyaji Y. Department of Urology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan e-mail: tsushima@med.okayama-u.ac.jp Tel.: +81 86 235 7285; Fax: +81 86 231 3986, JP aut NATIONALLICENCE 700 1- Murakami T. Department of Urology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan e-mail: tsushima@med.okayama-u.ac.jp Tel.: +81 86 235 7285; Fax: +81 86 231 3986, JP aut NATIONALLICENCE 700 1- Kumon H. Department of Urology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan e-mail: tsushima@med.okayama-u.ac.jp Tel.: +81 86 235 7285; Fax: +81 86 231 3986, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer