caa a22 4500 475742524 CHVBK 20180406123503.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s002400000138 doi (NATIONALLICENCE)springer-10.1007/s002400000138 Effects of cotinine on sperm motility, membrane function, and fertilizing capacity in vitro [Elektronische Daten] [Nikolaos Sofikitis, Manami Takenaka, Nikolaos Kanakas, Hlias Papadopoulos, Yasuhisa Yamamoto, Peter Drakakis, Ikuo Miyagawa] We evaluated the effect of cotinine on sperm fertilizing capacity in vitro. Human spermatozoa were washed and re-suspended in medium containing albumin and various concentrations of cotinine (0, 100, 200, 400, or 800 ng/ml). After an 8-h incubation period, sperm motility, hypoosmotic swelling test (HOST) outcome, and the percentage of hyperactivated spermatozoa were assayed. Aliquots of spermatozoa were then processed for the zona-free hamster oocyte sperm penetration assay (SPA) or hamster ooplasmic injections. Spermatozoa exposed to concentrations of cotinine equal to 400 or 800 ng/ml demonstrated significantly smaller outcomes for all of the above with the exception of after hamster ooplasmic injections, where high cotinine concentrations did not affect sperm viability or sperm capacity to undergo decondensation and activate hamster oocytes. It appears that cotinine concentrations of 400 or 800 ng/ml exert a detrimental effect on sperm motility, membrane function, and the ability to undergo capacitation. In addition, the current findings suggest that smokers with a high seminal plasma cotinine concentration who participate in assisted reproduction programs may be treated with intracytoplasmic sperm injections (ICSI) rather than conventional in vitro fertilization (IVF) trials. Springer-Verlag Berlin Heidelberg, 2000 Key words Smoking nationallicence Cotinine nationallicence Sperm nationallicence Fertilization nationallicence Oocyte activation nationallicence Sofikitis Nikolaos Department of Urology, Tottori University School of Medicine, 36 Nishimachi, Yonago 683, Japan Fax: +81-859-229323, JP aut Takenaka Manami Department of Urology, Tottori University School of Medicine, 36 Nishimachi, Yonago 683, Japan Fax: +81-859-229323, JP aut Kanakas Nikolaos Department of Urology, Tottori University School of Medicine, 36 Nishimachi, Yonago 683, Japan Fax: +81-859-229323, JP aut Papadopoulos Hlias Department of Urology, Tottori University School of Medicine, 36 Nishimachi, Yonago 683, Japan Fax: +81-859-229323, JP aut Yamamoto Yasuhisa Department of Urology, Tottori University School of Medicine, 36 Nishimachi, Yonago 683, Japan Fax: +81-859-229323, JP aut Drakakis Peter Department of Urology, Tottori University School of Medicine, 36 Nishimachi, Yonago 683, Japan Fax: +81-859-229323, JP aut Miyagawa Ikuo Department of Urology, Tottori University School of Medicine, 36 Nishimachi, Yonago 683, Japan Fax: +81-859-229323, JP aut https://doi.org/10.1007/s002400000138 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002400000138 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sofikitis Nikolaos Department of Urology, Tottori University School of Medicine, 36 Nishimachi, Yonago 683, Japan Fax: +81-859-229323, JP aut NATIONALLICENCE 700 1- Takenaka Manami Department of Urology, Tottori University School of Medicine, 36 Nishimachi, Yonago 683, Japan Fax: +81-859-229323, JP aut NATIONALLICENCE 700 1- Kanakas Nikolaos Department of Urology, Tottori University School of Medicine, 36 Nishimachi, Yonago 683, Japan Fax: +81-859-229323, JP aut NATIONALLICENCE 700 1- Papadopoulos Hlias Department of Urology, Tottori University School of Medicine, 36 Nishimachi, Yonago 683, Japan Fax: +81-859-229323, JP aut NATIONALLICENCE 700 1- Yamamoto Yasuhisa Department of Urology, Tottori University School of Medicine, 36 Nishimachi, Yonago 683, Japan Fax: +81-859-229323, JP aut NATIONALLICENCE 700 1- Drakakis Peter Department of Urology, Tottori University School of Medicine, 36 Nishimachi, Yonago 683, Japan Fax: +81-859-229323, JP aut NATIONALLICENCE 700 1- Miyagawa Ikuo Department of Urology, Tottori University School of Medicine, 36 Nishimachi, Yonago 683, Japan Fax: +81-859-229323, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer