caa a22 4500 475742532 CHVBK 20180406123503.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s002400000143 doi (NATIONALLICENCE)springer-10.1007/s002400000143 Expression of MAGE antigens and analysis of the inflammatory T-cell infiltrate in human seminoma [Elektronische Daten] [Rainer Grobholz, Caroline S. Verbeke, Christiane Schleger, Kai-Uwe Köhrmann, Beatrix Hein, Georg Wolf, Uwe Bleyl, Giulio C. Spagnoli, Keren Coplan, Denise Kolb, Kristin Iversen, Achim A. Jungbluth] The MAGE gene family encodes antigens that are recognized by cytotoxic T-cells. The expression of MAGE antigens has been linked to tumor stage, and MAGE peptides are under investigation as possible vaccines. Seminomas are tumors that are typically accompanied by a heavy inflammatory infiltrate, but have not been studied with regard to their MAGE antigen expression and its correlation with the inflammatory infiltrate. We investigated, therefore, MAGE protein expression, the amount of cytotoxic T-cells, clonality of the lymphocytic infiltrate, apoptotic activity and occurrence of necrosis. Specimens of 27 patients with classical seminoma were examined by immunohistochemistry for CD4, CD8, CD56, CD45R0, β2-microglobulin and HLA-DR. MAGE expression was detected with the monoclonal antibody 57B, reactive with MAGE-1, -3, -4, -6 and -12. Clonality of the inflammatory infiltrate was examined by multiplex polymerase chain reaction (PCR) analysis of the T-cell receptor rearrangement. Apoptotic cells were detected by DNA nick-end labeling of fragmented DNA, and the apoptotic index was determined semi-quantitatively. Expression of 57B was found in 19 (70%) of 27 seminomas. In all cases, more than 70% of T-cells expressed CD45R0. In four cases, a predominant infiltration of CD8-positive cytotoxic T-cells (CD4/CD8 ratio <1) was present. However, 15 seminomas showed a CD4/CD8 ratio >1. In all cases, infiltration of CD56-positive natural killer cells was only focal. HLA-DR expression was not detectable in tumor tissue; β2-microglobulin was only focal in three cases. Analysis of the T-cell clonality revealed a polyclonal population. The apoptotic index was not significantly different in 57B-positive seminomas (4.15%) compared with 57B negative seminomas (3.80%). Also, no correlation between the 57B expression and the occurrence of necrosis was found. MAGE antigens are homogeneously expressed in most seminomas, but their presence does not appear to represent a dominant epitope responsible for the lymphocytic infiltrate. Springer-Verlag Berlin Heidelberg, 2000 Key words MAGE nationallicence Seminoma nationallicence Tumor-infiltrating lymphocytes nationallicence Grobholz Rainer Department of Pathology, Ruprecht-Karls University Heidelberg, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany e-mail: rainer.grobholz@path.ma.uni-heidelberg.de Tel.: +49-621-383-3505; Fax: +49-621-383-2005, DE aut Verbeke Caroline S. Department of Pathology, Ruprecht-Karls University Heidelberg, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany e-mail: rainer.grobholz@path.ma.uni-heidelberg.de Tel.: +49-621-383-3505; Fax: +49-621-383-2005, DE aut Schleger Christiane Department of Pathology, Ruprecht-Karls University Heidelberg, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany e-mail: rainer.grobholz@path.ma.uni-heidelberg.de Tel.: +49-621-383-3505; Fax: +49-621-383-2005, DE aut Köhrmann Kai-Uwe Department of Urology, Ruprecht-Karls University Heidelberg, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany, DE aut Hein Beatrix Department of Pathology, Ruprecht-Karls University Heidelberg, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany e-mail: rainer.grobholz@path.ma.uni-heidelberg.de Tel.: +49-621-383-3505; Fax: +49-621-383-2005, DE aut Wolf Georg Department of Pathology, Ruprecht-Karls University Heidelberg, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany e-mail: rainer.grobholz@path.ma.uni-heidelberg.de Tel.: +49-621-383-3505; Fax: +49-621-383-2005, DE aut Bleyl Uwe Department of Pathology, Ruprecht-Karls University Heidelberg, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany e-mail: rainer.grobholz@path.ma.uni-heidelberg.de Tel.: +49-621-383-3505; Fax: +49-621-383-2005, DE aut Spagnoli Giulio C. Department of Surgery and Research, University of Basel, Hebelstrasse 20, 4031 Basel, Switzerland, CH aut Coplan Keren Ludwig Institute for Cancer Research at Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA, US aut Kolb Denise Ludwig Institute for Cancer Research at Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA, US aut Iversen Kristin Ludwig Institute for Cancer Research at Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA, US aut Jungbluth Achim A. Ludwig Institute for Cancer Research at Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA, US aut https://doi.org/10.1007/s002400000143 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002400000143 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Grobholz Rainer Department of Pathology, Ruprecht-Karls University Heidelberg, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany e-mail: rainer.grobholz@path.ma.uni-heidelberg.de Tel.: +49-621-383-3505; Fax: +49-621-383-2005, DE aut NATIONALLICENCE 700 1- Verbeke Caroline S. Department of Pathology, Ruprecht-Karls University Heidelberg, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany e-mail: rainer.grobholz@path.ma.uni-heidelberg.de Tel.: +49-621-383-3505; Fax: +49-621-383-2005, DE aut NATIONALLICENCE 700 1- Schleger Christiane Department of Pathology, Ruprecht-Karls University Heidelberg, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany e-mail: rainer.grobholz@path.ma.uni-heidelberg.de Tel.: +49-621-383-3505; Fax: +49-621-383-2005, DE aut NATIONALLICENCE 700 1- Köhrmann Kai-Uwe Department of Urology, Ruprecht-Karls University Heidelberg, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany, DE aut NATIONALLICENCE 700 1- Hein Beatrix Department of Pathology, Ruprecht-Karls University Heidelberg, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany e-mail: rainer.grobholz@path.ma.uni-heidelberg.de Tel.: +49-621-383-3505; Fax: +49-621-383-2005, DE aut NATIONALLICENCE 700 1- Wolf Georg Department of Pathology, Ruprecht-Karls University Heidelberg, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany e-mail: rainer.grobholz@path.ma.uni-heidelberg.de Tel.: +49-621-383-3505; Fax: +49-621-383-2005, DE aut NATIONALLICENCE 700 1- Bleyl Uwe Department of Pathology, Ruprecht-Karls University Heidelberg, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany e-mail: rainer.grobholz@path.ma.uni-heidelberg.de Tel.: +49-621-383-3505; Fax: +49-621-383-2005, DE aut NATIONALLICENCE 700 1- Spagnoli Giulio C. Department of Surgery and Research, University of Basel, Hebelstrasse 20, 4031 Basel, Switzerland, CH aut NATIONALLICENCE 700 1- Coplan Keren Ludwig Institute for Cancer Research at Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA, US aut NATIONALLICENCE 700 1- Kolb Denise Ludwig Institute for Cancer Research at Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA, US aut NATIONALLICENCE 700 1- Iversen Kristin Ludwig Institute for Cancer Research at Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA, US aut NATIONALLICENCE 700 1- Jungbluth Achim A. Ludwig Institute for Cancer Research at Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer