caa a22 4500 47574263X CHVBK 20180406123504.0 cr unu---uuuuu 170329e20000601xx s 000 0 eng 10.1007/s002400000103 doi (NATIONALLICENCE)springer-10.1007/s002400000103 Expression of the SART1 tumor rejection antigen in renal cell carcinoma [Elektronische Daten] [I. Shintaku, N. Kawagoe, S. Yutani, S. Hoshi, S. Orikasa, O. Yoshizumi, K. Itoh] We have previously described the SART1 gene, which encodes both the SART1259 antigen expressed in the cytosol of the majority of squamous cell carcinomas and some adenocarcinomas and the SART1800 antigen expressed in the nucleus of the majority of proliferating cells. The SART1259 antigen is recognized by HLA-A24 and A26-restricted cytotoxic T lymphocytes (CTLs). The present study investigated the expression of these two antigens in renal cell carcinomas (RCCs) in order to identify an appropriate molecule for use in specific immunotherapy for RCC patients. These two antigens were detected in all RCC cell lines and cells of the primary cultures of the RCCs tested. Further, they were detectable in cells of the primary cultures of non-tumorous kidney tissues. In contrast to these cultured cells, SART1259 was detectable in only a few uncultured RCC tissues (5/20, 25%) and was undetectable in non-tumorous kidney tissues. SART1800 was also scarcely detectable in uncultured RCC tissues (3/20, 15%) and non-tumorous kidney tissues (4/20, 20%). HLA-A2402-restricted and tumor-specific CTL (KE4-CTL) used for the cloning of the SART1 gene showed significant levels of cytotoxicity to both the cells from the RCC cell line and the cells from the primary cultures of RCC tissues, but did not lyse any normal cells, including cells from the primary cultures of non-tumorous kidney tissues. The SART1-derived peptide at positions 690-698 induced HLA-A24 restricted CTLs cytotoxic to RCC cells from peripheral blood mononuclear cells (PBMCs) of RCC patients. Therefore, the SART1 peptide could be an appropriate molecule for use in peptide-based specific immunotherapy for RCC patients. Springer-Verlag Berlin Heidelberg, 2000 Key words SART1 antigen nationallicence Renal cell carcinoma nationallicence Cytotoxic T lymphocyte nationallicence Immunotherapy nationallicence Cancer vaccine nationallicence Shintaku I. Department of Urology, Tohoku University, School of Medicine, 1-1 Seiryomachi, Aoba-ku, Sendai, 980-8574, Japan e-mail: shintaku@uro.med.tohoku.ac.jp Tel.:+81-22-717-7278, Fax: +81-22-717-7283, JP aut Kawagoe N. Department of Urology, Kurume University, School of Medicine, Kurume, Japan, JP aut Yutani S. Department of Immunology, Kurume University, School of Medicine, Kurume, Japan, JP aut Hoshi S. Department of Urology, Tohoku University, School of Medicine, 1-1 Seiryomachi, Aoba-ku, Sendai, 980-8574, Japan e-mail: shintaku@uro.med.tohoku.ac.jp Tel.:+81-22-717-7278, Fax: +81-22-717-7283, JP aut Orikasa S. Department of Urology, Tohoku University, School of Medicine, 1-1 Seiryomachi, Aoba-ku, Sendai, 980-8574, Japan e-mail: shintaku@uro.med.tohoku.ac.jp Tel.:+81-22-717-7278, Fax: +81-22-717-7283, JP aut Yoshizumi O. St. Mary Hospital, Kurume, Japan, JP aut Itoh K. Department of Immunology, Kurume University, School of Medicine, Kurume, Japan, JP aut https://doi.org/10.1007/s002400000103 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002400000103 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shintaku I. Department of Urology, Tohoku University, School of Medicine, 1-1 Seiryomachi, Aoba-ku, Sendai, 980-8574, Japan e-mail: shintaku@uro.med.tohoku.ac.jp Tel.:+81-22-717-7278, Fax: +81-22-717-7283, JP aut NATIONALLICENCE 700 1- Kawagoe N. Department of Urology, Kurume University, School of Medicine, Kurume, Japan, JP aut NATIONALLICENCE 700 1- Yutani S. Department of Immunology, Kurume University, School of Medicine, Kurume, Japan, JP aut NATIONALLICENCE 700 1- Hoshi S. Department of Urology, Tohoku University, School of Medicine, 1-1 Seiryomachi, Aoba-ku, Sendai, 980-8574, Japan e-mail: shintaku@uro.med.tohoku.ac.jp Tel.:+81-22-717-7278, Fax: +81-22-717-7283, JP aut NATIONALLICENCE 700 1- Orikasa S. Department of Urology, Tohoku University, School of Medicine, 1-1 Seiryomachi, Aoba-ku, Sendai, 980-8574, Japan e-mail: shintaku@uro.med.tohoku.ac.jp Tel.:+81-22-717-7278, Fax: +81-22-717-7283, JP aut NATIONALLICENCE 700 1- Yoshizumi O. St. Mary Hospital, Kurume, Japan, JP aut NATIONALLICENCE 700 1- Itoh K. Department of Immunology, Kurume University, School of Medicine, Kurume, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer