caa a22 4500 475742656 CHVBK 20180406123504.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1007/s002400000128 doi (NATIONALLICENCE)springer-10.1007/s002400000128 Renal tubular apoptosis after complete ureteral obstruction in the presence of hyperoxaluria [Elektronische Daten] [Tahir Turan, Ömer Levent Tuncay, Alp Usubütün, Tarik Yonguç, Zafer Aybek, Özcan Atahan] Hyperoxaluria is a well-known cause of renal stone disease and in vitro studies have shown that oxalate crystals have a stimulatory effect on apoptosis of renal tubular epithelial cells. Total and partial ureteral obstruction also have an accelerating effect on apoptosis of renal tubular epithelial cells. The aim of the present study was to investigate the apoptotic effect of unilateral ureteral obstruction in the presence of hyperoxaluria on the rat kidney. Twenty-eight male Wistar rats were divided into four groups, with seven rats in each. The groups were named G1 (control), G2 (hyperoxaluric), G3 (obstructive) and G4 (hyperoxaluric + obstructive). G2 and G4 rats were given 1% ethylene glycol (a precursor for oxalates) in their drinking water. G1 and G2 rats underwent sham operation, while left proximal ureteral ligation with a 5-zero silk suture was performed on G3 and G4 animals. The rats were sacrificed 2 weeks after the operation; left nephrectomy was then performed. We searched for the apoptotic cells by direct immuno-peroxidase detection of digoxigenin-labeled genomic DNA. The mean ± SD values of the apoptotic cell count was 0.86 ± 0.90 in G1 and 4.33 ± 3.81 in G2. The values for G3 and G4 were 30.17 ± 16.85 and 302.67 ± 184.45, respectively. We found a statistically significant difference between all groups (P < 0.001). When compared with the control group (G1), the mean apoptotic cell count was fivefold that of G2 and 35- and 351-fold those of G3 and G4, respectively. Our study demonstrated that hyperoxaluria with complete ureteral obstruction induces an excessive level of apoptosis, which is responsible for renal damage, and that ureteral obstruction is a more important factor for apoptosis than hyperoxaluria. Considering these data, we also believe that research studies for medical preventive measures must be considered for patients with ureteral obstruction and/or hyperoxaluria. Springer-Verlag Berlin Heidelberg, 2000 Key words Kidney nationallicence Ureter nationallicence Urinary obstruction nationallicence Hydronephrosis nationallicence Hyperoxaluria nationallicence Apoptosis nationallicence Turan Tahir Department of Urology, Pamukkale University, School of Medicine, Denizli, Turkey, TR aut Tuncay Ömer Levent Department of Urology, Pamukkale University, School of Medicine, Denizli, Turkey, TR aut Usubütün Alp Department of Pathology, Hacettepe University, School of Medicine, Ankara, Turkey, TR aut Yonguç Tarik Department of Urology, Pamukkale University, School of Medicine, Denizli, Turkey, TR aut Aybek Zafer Department of Urology, Pamukkale University, School of Medicine, Denizli, Turkey, TR aut Atahan Özcan Department of Urology, Pamukkale University, School of Medicine, Denizli, Turkey, TR aut https://doi.org/10.1007/s002400000128 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002400000128 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Turan Tahir Department of Urology, Pamukkale University, School of Medicine, Denizli, Turkey, TR aut NATIONALLICENCE 700 1- Tuncay Ömer Levent Department of Urology, Pamukkale University, School of Medicine, Denizli, Turkey, TR aut NATIONALLICENCE 700 1- Usubütün Alp Department of Pathology, Hacettepe University, School of Medicine, Ankara, Turkey, TR aut NATIONALLICENCE 700 1- Yonguç Tarik Department of Urology, Pamukkale University, School of Medicine, Denizli, Turkey, TR aut NATIONALLICENCE 700 1- Aybek Zafer Department of Urology, Pamukkale University, School of Medicine, Denizli, Turkey, TR aut NATIONALLICENCE 700 1- Atahan Özcan Department of Urology, Pamukkale University, School of Medicine, Denizli, Turkey, TR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer