caa a22 4500 475742664 CHVBK 20180406123504.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1007/s002400000114 doi (NATIONALLICENCE)springer-10.1007/s002400000114 Determination of mouse bladder inflammatory response to E. coli lipopolysaccharide [Elektronische Daten] [T. J. Jerde, D. E. Bjorling, H. Steinberg, T. Warner, R. Saban] Evaluation of the severity of histologic changes associated with cystitis is often subjective and inconsistent from one sample to the next. The objective of this study was to establish a consistent, reproducible method to quantify histologic changes in a mouse model of lipopolysaccharide (LPS)-induced cystitis. Either LPS (n=8) or pyrogen-free saline (n=8) was instilled intravesically into the bladders of female C57bk-6 J mice. Twenty-four hours later, mice in these groups as well as eight untreated controls were sacrificed and bladders were removed, fixed in formalin, and stained with hematoxylin and eosin (H&E). A bladder inflammatory index (BII) was described by reviewing tissues for edema, leukocyte infiltration, and hemorrhage. Cross-sections were evaluated by a single pathologist in a blinded manner based on the objective BII described. The BII method for objectively analyzing bladder inflammation was effective and reproducible. Bladders instilled with LPS had significantly increased inflammation scores for edema, leukocyte infiltration, and hemorrhage compared with those instilled with saline or untreated controls (n=8, P < 0.05). These results demonstrate that LPS causes bladder inflammation when instilled intravesically and that inflammation of mouse bladders can be objectively quantified using the histological method described. Springer-Verlag Berlin Heidelberg, 2000 Key words Bladder nationallicence Inflammation nationallicence Histology nationallicence Lipopolysaccharide nationallicence Jerde T. J. Department of Surgery-Division of Urology, University of Wisconsin Medical School, Madison, WI USA, US aut Bjorling D. E. University of Wisconsin-School of Veterinary Medicine, Department of Surgical Sciences, 2023 School of Veterinary Medicine, 2015 Linden Drive West, Madison, WI 53706, USA e-mail: bjorlind@svm.vetmed.wisc.edu Tel.: +608-263-4808; Fax: +1 608-263-7930, US aut Steinberg H. Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA, US aut Warner T. Department of Surgical Pathology, University of Wisconsin Medical School, Madison, WI, USA, US aut Saban R. Enteric Neuromuscular Diseases Laboratory, Division of Gastroenterology, University of Texas, Medical Branch, Galveston, TX, USA, US aut https://doi.org/10.1007/s002400000114 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002400000114 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jerde T. J. Department of Surgery-Division of Urology, University of Wisconsin Medical School, Madison, WI USA, US aut NATIONALLICENCE 700 1- Bjorling D. E. University of Wisconsin-School of Veterinary Medicine, Department of Surgical Sciences, 2023 School of Veterinary Medicine, 2015 Linden Drive West, Madison, WI 53706, USA e-mail: bjorlind@svm.vetmed.wisc.edu Tel.: +608-263-4808; Fax: +1 608-263-7930, US aut NATIONALLICENCE 700 1- Steinberg H. Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA, US aut NATIONALLICENCE 700 1- Warner T. Department of Surgical Pathology, University of Wisconsin Medical School, Madison, WI, USA, US aut NATIONALLICENCE 700 1- Saban R. Enteric Neuromuscular Diseases Laboratory, Division of Gastroenterology, University of Texas, Medical Branch, Galveston, TX, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer