caa a22 4500 475742753 CHVBK 20180406123504.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1007/s002400000130 doi (NATIONALLICENCE)springer-10.1007/s002400000130 Inhibition by Vitamin C of DNA adduct formation and arylamine N -acetyltransferase activity in human bladder tumor cells [Elektronische Daten] [H. C. Wu, H. F. Lu, C. F. Hung, J. G. Chung] Previous studies have already demonstrated the protective role of vitamin C (ascorbic acid) in certain types of cancer. This study reports on the effects of vitamin C on arylamineN-acetyltransferase (NAT) activity and DNA adduct formation in a human bladder tumor cell (T24) line. The activity of NAT was measured using high-performance liquid chromatography (HPLC), by assaying for the amounts of acetylated 2-aminofluorene (AF) and p-aminobenzoic acid (PABA) and the remaining amounts of AF and PABA. T24 cells were used for examining NAT activity and carcinogen-DNA adduct formation. The results demonstrated that NAT activity and 2-aminofluorene-DNA adduct formation in T24 cells were inhibited and decreased by vitamin C in a dose-dependent manner. The apparent kinetic parameters (apparent values ofK m and V max) from T24 cells were also determined with and without vitamin C cotreatment. The data also indicated that vitamin C decreased the apparent values of K m and V max from T24 cells. Springer-Verlag Berlin Heidelberg, 2000 Key words Vitamin C nationallicence N-acetyltransferase nationallicence 2-aminofluorene nationallicence DNA adduct nationallicence Bladder cancer nationallicence Wu H. C. Department of Urology, China Medical College Hospital, No 2, Yuh-Der Road, Taichung 400, Taiwan, Republic of China, CN aut Lu H. F. Department of Urology, China Medical College Hospital, No 2, Yuh-Der Road, Taichung 400, Taiwan, Republic of China, CN aut Hung C. F. Department of Surgery, Jen-Ai Hospital, No 483, Tong-Rong Road, Tali, Taichung 400, Taiwan, Republic of China, CN aut Chung J. G. Department of Microbiology, China Medical College, 91 Hsueh-Shih Road, Taichung 400, Taiwan, Republic of China, CN aut https://doi.org/10.1007/s002400000130 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002400000130 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wu H. C. Department of Urology, China Medical College Hospital, No 2, Yuh-Der Road, Taichung 400, Taiwan, Republic of China, CN aut NATIONALLICENCE 700 1- Lu H. F. Department of Urology, China Medical College Hospital, No 2, Yuh-Der Road, Taichung 400, Taiwan, Republic of China, CN aut NATIONALLICENCE 700 1- Hung C. F. Department of Surgery, Jen-Ai Hospital, No 483, Tong-Rong Road, Tali, Taichung 400, Taiwan, Republic of China, CN aut NATIONALLICENCE 700 1- Chung J. G. Department of Microbiology, China Medical College, 91 Hsueh-Shih Road, Taichung 400, Taiwan, Republic of China, CN aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer