caa a22 4500 475742761 CHVBK 20180406123504.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1007/s002400000127 doi (NATIONALLICENCE)springer-10.1007/s002400000127 An in vitro model for videoimaging of human bladder smooth muscle cell contractions [Elektronische Daten] [Stefan Corvin, Simone T. Bösch, Christoph Maneschg, Georg Bartsch, Helmut Klocker] Knowledge regarding human bladder smooth muscle cell (SMC) physiology is very limited. Only a few specific medical therapies for bladder disorders have therefore been established. The objective of this study was to develop a model for videomicroscopy of bladder SMC contractions. Cells were isolated from human cystoprostatectomy specimens and cultured in a modified EMEM medium. These cells were identified as SMCs by means of immunohistochemistry. For videomicroscopy, the culture flasks were coated with a viscous agent to allow cell contraction. Contractions were visualized by means of a cell culture microscope with a time-lapse videosystem. For cholinergic stimulation of the cells, acetylcholine, in concentrations ranging from 100 μM to 10 mM, was applied. The percentage of contracting cells within the observation field was evaluated for quantitative analysis. In control experiments without contractile stimulant 6% of the cells were observed to contract. Stimulation with acetylcholine induced a significant dose-dependent increase to 47% in contracting cells. These results demonstrated that videomicroscopy is an appropriate tool to investigate the contraction mechanisms of bladder SMCs. This model offers the possibility of studying drug effects on the human detrusor in vitro. Springer-Verlag Berlin Heidelberg, 2000 Key words Videomicroscopy nationallicence Smooth muscle cell nationallicence Contraction nationallicence Acetylcholine nationallicence Corvin Stefan Department of Urology, Ludwig-Maximilians-Universität, Marchioninistrasse 15, D-81377 Munich, Germany e-mail: Stefan.Corvin@uro.med.uni-muenchen.de Tel.: +49-89-7095-3530; Fax: +49-89-70958890, DE aut Bösch Simone T. Department of Urology, University of Innsbruck, Austria, AT aut Maneschg Christoph Department of Urology, University of Innsbruck, Austria, AT aut Bartsch Georg Department of Urology, University of Innsbruck, Austria, AT aut Klocker Helmut Department of Urology, University of Innsbruck, Austria, AT aut https://doi.org/10.1007/s002400000127 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002400000127 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Corvin Stefan Department of Urology, Ludwig-Maximilians-Universität, Marchioninistrasse 15, D-81377 Munich, Germany e-mail: Stefan.Corvin@uro.med.uni-muenchen.de Tel.: +49-89-7095-3530; Fax: +49-89-70958890, DE aut NATIONALLICENCE 700 1- Bösch Simone T. Department of Urology, University of Innsbruck, Austria, AT aut NATIONALLICENCE 700 1- Maneschg Christoph Department of Urology, University of Innsbruck, Austria, AT aut NATIONALLICENCE 700 1- Bartsch Georg Department of Urology, University of Innsbruck, Austria, AT aut NATIONALLICENCE 700 1- Klocker Helmut Department of Urology, University of Innsbruck, Austria, AT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer