caa a22 4500 475742877 CHVBK 20180406123504.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s002400000123 doi (NATIONALLICENCE)springer-10.1007/s002400000123 The connective tissue framework in the normal prostate, B.P.H and prostate cancer: analysis by scanning electron microscopy after cellular digestion [Elektronische Daten] [Colin Morrison, John Thornhill, Eoin Gaffney] The objective of this study was twofold: (1) to determine if a cellular digestion process can facilitate examination of the morphology of the connective tissue framework of the prostate, and (2) to examine the connective tissue framework in normal prostate tissue, benign prostatic hyperplasia (BPH) and prostate cancer. Ten prostate glands were examined. Using the Ohtani method of digestion, the cellular elements were removed. This enabled scanning electron microscopy analysis of the connective tissue framework within the prostatic tissue. Light microscopy of tissue blocks determined the histology of specimens. The prostate is supported by a highly structured network of collagen fibres. This network of fibres varies in normal and diseased states. In benign prostatic hyperplasia, the collagen network is dense, with an increased number of fibres. In prostatic adenocarcinoma, there is non-uniform swelling with a loss and disintegration of collagen fibres. In conclusion, sodium hydroxide cellular digestion provides an excellent method for demonstrating the connective tissue framework of prostatic tissue. The morphological changes in collagen fibres in normal prostate, benign prostatic hyperplasia and prostatic adenocarcinoma have implications for prostate growth in normal and diseased states. Springer-Verlag Berlin Heidelberg, 2000 Key words Connective tissue nationallicence Prostate nationallicence Benign prostatic hyperplasia nationallicence Cancer nationallicence Scanning electron microscopy nationallicence Morrison Colin Urology Department, The Adelaide & Meath Hospital, Dublin Incorporating The National Children's Hospital, Tallaght, Dublin 24, Ireland Tel.: +353-1-4142000; Fax: +353-1-4143863, IE aut Thornhill John Urology Department, The Adelaide & Meath Hospital, Dublin Incorporating The National Children's Hospital, Tallaght, Dublin 24, Ireland Tel.: +353-1-4142000; Fax: +353-1-4143863, IE aut Gaffney Eoin Histopathology Department, St. James's Hospital, Dublin 8, Ireland, IE aut https://doi.org/10.1007/s002400000123 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002400000123 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Morrison Colin Urology Department, The Adelaide & Meath Hospital, Dublin Incorporating The National Children's Hospital, Tallaght, Dublin 24, Ireland Tel.: +353-1-4142000; Fax: +353-1-4143863, IE aut NATIONALLICENCE 700 1- Thornhill John Urology Department, The Adelaide & Meath Hospital, Dublin Incorporating The National Children's Hospital, Tallaght, Dublin 24, Ireland Tel.: +353-1-4142000; Fax: +353-1-4143863, IE aut NATIONALLICENCE 700 1- Gaffney Eoin Histopathology Department, St. James's Hospital, Dublin 8, Ireland, IE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer