caa a22 4500 475742907 CHVBK 20180406123504.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s002400000131 doi (NATIONALLICENCE)springer-10.1007/s002400000131 Expression of cadherins and catenins in paired tumor and non-neoplastic primary prostate cultures and corresponding prostatectomy specimens [Elektronische Daten] [Jianzhou Wang, Diane Krill, Michael Torbenson, Qi Wang, Michelle Bisceglia, Judy Stoner, Angela Thomas, Petrina DeFlavia, Rajiv Dhir, Michael J. Becich] Cadherins are a family of transmembrane proteins that play a crucial role in cell differentiation, cell migration, and intercellular adhesion. Cadherins are associated with catenins through their highly conserved cytoplasmic domain. Down-regulation of E-cadherin protein has been shown in various human cancers. This study examined the expression of cadherins and associated catenins at the mRNA level. Paired tumor and non-neoplastic primary prostate cultures were obtained from surgical specimens. Quantitative multiplex fluorescence reverse transcriptase-polymerase chain reaction (QMF RT-PCR) and quantitative analysis were performed and correlated with immunostain results. Six of seven cases of neoplastic cultures showed moderately-to-markedly decreased levels of E-cadherin and P-cadherin mRNA. Similar losses of α-catenin and β-catenin mRNA were also observed. The results of QMF RT-PCR showed good correlation with the results of immunohistochemical studies based on corresponding formalin-fixed sections. In conclusion, this paper presents a coordinated down-regulation in the expression of E-cadherin and associated catenins at the mRNA and protein level in most of the cases studied. This down-regulation may play an important role in the pathogenesis of prostate cancer. Springer-Verlag Berlin Heidelberg, 2000 Key words Prostate adenocarcinoma nationallicence Cadherin nationallicence Catenin nationallicence Adhesion molecules nationallicence Wang Jianzhou Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA aut Krill Diane Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA aut Torbenson Michael Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA aut Wang Qi Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA aut Bisceglia Michelle Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA aut Stoner Judy Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA aut Thomas Angela Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA aut DeFlavia Petrina Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA aut Dhir Rajiv Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA aut Becich Michael J. Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA aut https://doi.org/10.1007/s002400000131 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002400000131 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Jianzhou Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA aut NATIONALLICENCE 700 1- Krill Diane Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA aut NATIONALLICENCE 700 1- Torbenson Michael Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA aut NATIONALLICENCE 700 1- Wang Qi Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA aut NATIONALLICENCE 700 1- Bisceglia Michelle Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA aut NATIONALLICENCE 700 1- Stoner Judy Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA aut NATIONALLICENCE 700 1- Thomas Angela Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA aut NATIONALLICENCE 700 1- DeFlavia Petrina Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA aut NATIONALLICENCE 700 1- Dhir Rajiv Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA aut NATIONALLICENCE 700 1- Becich Michael J. Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer