caa a22 4500 475742915 CHVBK 20180406123504.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s002400050144 doi (NATIONALLICENCE)springer-10.1007/s002400050144 Pulsed-field gel electrophoresis for the analysis of Listeria monocytogenes infection clusters after kidney transplantation [Elektronische Daten] [C. Reek, W. Tenschert, H-A. Elsner, P-M. Kaulfers, H. Huland] Listeria monocytogenes causes a rare, life-threatening infection in recipients of transplanted organs. We used cultures of blood and cerebrospinal fluid to characterize isolates and to distinguish cases in clusters from what might have been sporadic cases. From December 1994 to November 1995, six systemic L. monocytogenes infections occurred at our renal-transplantation unit. We confirmed the clinical diagnosis with blood and cerebrospinal fluid cultures and characterized the isolates retrospectively with pulsed-field gel electrophoresis (PFGE), phage-typing, and serotyping. We also performed an environmental investigation (food, drug, and stool). We took samples after the first two L. monocytogenes infections and then after cases three and four occurred. All patients recovered completely, and no graft was lost. Four patients had identical or genetically related L. monocytogenes isolates in PFGE (type A) and serotyping (type 1/2b). The other two had PFGE type B and G. L. monocytogenes was not detected in food or drug samples from patients on the renal- transplantation ward or in stool samples from the ward staff. It was concluded that PFGE allows sporadic cases and cluster cases of L. monocytogenes infection to be distinguished. Springer-Verlag Berlin Heidelberg, 2000 Key words Renal transplantation nationallicence Listeria monocytogenes nationallicence Nosocomial infection nationallicence Pulsed-field gel electrophoresis (PFGE) nationallicence Reek C. Urologische Klinik und Poliklinik des Universitäts Krankenhaus Eppendorf, Martinistrasses 52, 20246 Hamburg, University of Hamburg Eppendorf, Germany Tel.: +49 40 42803 4462, Fax: +49 40 42803 6887, DE aut Tenschert W. Urologische Klinik und Poliklinik des Universitäts Krankenhaus Eppendorf, Martinistrasses 52, 20246 Hamburg, University of Hamburg Eppendorf, Germany Tel.: +49 40 42803 4462, Fax: +49 40 42803 6887, DE aut Elsner H-A Department of Microbiology and Immunology, University of Hamburg Eppendorf, Germany, DE aut Kaulfers P-M Department of Microbiology and Immunology, University of Hamburg Eppendorf, Germany, DE aut Huland H. Urologische Klinik und Poliklinik des Universitäts Krankenhaus Eppendorf, Martinistrasses 52, 20246 Hamburg, University of Hamburg Eppendorf, Germany Tel.: +49 40 42803 4462, Fax: +49 40 42803 6887, DE aut https://doi.org/10.1007/s002400050144 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002400050144 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Reek C. Urologische Klinik und Poliklinik des Universitäts Krankenhaus Eppendorf, Martinistrasses 52, 20246 Hamburg, University of Hamburg Eppendorf, Germany Tel.: +49 40 42803 4462, Fax: +49 40 42803 6887, DE aut NATIONALLICENCE 700 1- Tenschert W. Urologische Klinik und Poliklinik des Universitäts Krankenhaus Eppendorf, Martinistrasses 52, 20246 Hamburg, University of Hamburg Eppendorf, Germany Tel.: +49 40 42803 4462, Fax: +49 40 42803 6887, DE aut NATIONALLICENCE 700 1- Elsner H-A Department of Microbiology and Immunology, University of Hamburg Eppendorf, Germany, DE aut NATIONALLICENCE 700 1- Kaulfers P-M Department of Microbiology and Immunology, University of Hamburg Eppendorf, Germany, DE aut NATIONALLICENCE 700 1- Huland H. Urologische Klinik und Poliklinik des Universitäts Krankenhaus Eppendorf, Martinistrasses 52, 20246 Hamburg, University of Hamburg Eppendorf, Germany Tel.: +49 40 42803 4462, Fax: +49 40 42803 6887, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer