caa a22 4500 475742923 CHVBK 20180406123505.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s002400050153 doi (NATIONALLICENCE)springer-10.1007/s002400050153 Real-time monitoring of nitric oxide in ischemia-reperfusion rat kidney [Elektronische Daten] [M. Saito, I. Miyagawa] In this study we attempted to clarify the release of nitric oxide (NO) and its role in the ischemia-reperfusion rat kidney. After right nephrectomy, male Wistar rats were divided into four groups: one sham operated and three groups who underwent ischemia (30 min) and reperfusion of the left renal artery. Thirty minutes prior to ischemia-reperfusion, two groups were injected intraperitoneally with 10 and 30 mg/kg of NG-nitro-l-arginine methylester (L-NAME). Real-time monitoring of blood flow and NO release in the rat kidney was measured with a laser Doppler flowmeter and an NO-selective electrode, respectively. Serum creatinine and blood urea nitrogen (BUN) levels were measured 1 and 7 days after the induction of ischemia-reperfusion. Clamping of the renal artery decreased blood flow to 1-5% of the basal level measured before clamping. After removal of the clip, the blood flow of the 30 mg/kg L-NAME rats was significantly lower than that of the controls. Immediately following the clipping of the renal artery, NO release rapidly increased. After removing the clip, NO release immediately returned to three-quarters of the basal level. Serum creatinine and BUN levels of the ischemia-reperfusion rats were slightly but not significantly higher and those of 30 mg L-NAME rats were significantly higher than those of the control or ischemia-reperfusion rats 1 day and 7 days after ischemia-reperfusion. Our data suggest that NO acts as a cytoprotective agent in ischemia-reperfusion injury of the rat kidney. Springer-Verlag Berlin Heidelberg, 2000 Key words Kidney nationallicence Nitric oxide nationallicence Ischemia-reperfusion injury nationallicence Rat nationallicence Saito M. Department of Urology, Faculty of Medicine, Tottori University, 36-1 Nishimachi, Yonago 683-0826, Japan, e-mail: saitomo@grape.med.tottori-u.ac.jp, Tel.: +81 859 34 8119; Fax: +81 859 34 8092, JP aut Miyagawa I. Department of Urology, Faculty of Medicine, Tottori University, 36-1 Nishimachi, Yonago 683-0826, Japan, e-mail: saitomo@grape.med.tottori-u.ac.jp, Tel.: +81 859 34 8119; Fax: +81 859 34 8092, JP aut https://doi.org/10.1007/s002400050153 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002400050153 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Saito M. Department of Urology, Faculty of Medicine, Tottori University, 36-1 Nishimachi, Yonago 683-0826, Japan, e-mail: saitomo@grape.med.tottori-u.ac.jp, Tel.: +81 859 34 8119; Fax: +81 859 34 8092, JP aut NATIONALLICENCE 700 1- Miyagawa I. Department of Urology, Faculty of Medicine, Tottori University, 36-1 Nishimachi, Yonago 683-0826, Japan, e-mail: saitomo@grape.med.tottori-u.ac.jp, Tel.: +81 859 34 8119; Fax: +81 859 34 8092, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer