caa a22 4500 475742958 CHVBK 20180406123505.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s002400050145 doi (NATIONALLICENCE)springer-10.1007/s002400050145 Induction of metallothionein synthesis with preservation of testicular function in rats following long term renal transplantation [Elektronische Daten] [L. Cai, D. X. Deng, J. Jiang, S. Chen, R. Zhong, M. G. Cherian, S. Chakrabarti] Metallothionein (MT), as an acute phase or stress-response protein and free radical scavenger, is related to inflammation and cellular protection from oxidative damage. In order to evaluate long-term testicular damage and the role of MT following renal transplant, nine allogenic (Fisher 344 → Lewis) and seven isogenic (Lewis → Lewis) renal transplants were performed and the recipient rats were followed for 140 days when allografts develop chronic transplant rejection. Testicular weight, light microscopic morphology, and lactate dehydrogenase-X enzyme activity were assessed. Testicular MT was determined by Cd-heme assay, and was localized immunocytochemically using a polyclonal rabbit antibody. No differences in testis weight, morphology, or LDH-X enzyme activity were found between allograft and isograft recipients. Testicular MT level was significantly increased in the testis of allograft recipients. Testicular zinc (Zn) and copper (Cu) levels, but not iron (Fe) level, were significantly higher in testis with allograft kidney than that with isograft kidney. In addition, Cu/Zn ratio was also significantly high in the allograft group. However, the MT level did not show any significant correlation either with Cu and Zn alone or with Cu/Zn and Fe/Zn ratios. These data suggest that allogenic stimuli may induce MT synthesis in the recipient testis. The increased MT level in an allograft may offer a protective action from oxidative damage in the testis. Springer-Verlag Berlin Heidelberg, 2000 Key words Renal transplantation nationallicence Metallothionein nationallicence Testicular function nationallicence Testicular metals nationallicence Cai L. Department of Pathology, Medical Science Building, The University of Western Ontario, London, N6A 5C1, Canada e-mail: schakrab@julian.uwo.ca Fax: +1 519 661 3370, CA aut Deng D. X. Department of Pathology, Medical Science Building, The University of Western Ontario, London, N6A 5C1, Canada e-mail: schakrab@julian.uwo.ca Fax: +1 519 661 3370, CA aut Jiang J. Department of Surgery, The University of Western Ontario, London, Canada, CA aut Chen S. Department of Pathology, Medical Science Building, The University of Western Ontario, London, N6A 5C1, Canada e-mail: schakrab@julian.uwo.ca Fax: +1 519 661 3370, CA aut Zhong R. Departments of Pathology, and Surgery, Microbiology and Immunology, The University of Western Ontario, Multi-Organ Transplant Program, London Health Sciences Center, London, Ontario, Canada, CA aut Cherian M. G. Departments of Pathology and Pharmacology and Toxicology, The University of Western Ontario, London, Canada, CA aut Chakrabarti S. Department of Pathology, Medical Science Building, The University of Western Ontario, London, N6A 5C1, Canada e-mail: schakrab@julian.uwo.ca Fax: +1 519 661 3370, CA aut https://doi.org/10.1007/s002400050145 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002400050145 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cai L. Department of Pathology, Medical Science Building, The University of Western Ontario, London, N6A 5C1, Canada e-mail: schakrab@julian.uwo.ca Fax: +1 519 661 3370, CA aut NATIONALLICENCE 700 1- Deng D. X. Department of Pathology, Medical Science Building, The University of Western Ontario, London, N6A 5C1, Canada e-mail: schakrab@julian.uwo.ca Fax: +1 519 661 3370, CA aut NATIONALLICENCE 700 1- Jiang J. Department of Surgery, The University of Western Ontario, London, Canada, CA aut NATIONALLICENCE 700 1- Chen S. Department of Pathology, Medical Science Building, The University of Western Ontario, London, N6A 5C1, Canada e-mail: schakrab@julian.uwo.ca Fax: +1 519 661 3370, CA aut NATIONALLICENCE 700 1- Zhong R. Departments of Pathology, and Surgery, Microbiology and Immunology, The University of Western Ontario, Multi-Organ Transplant Program, London Health Sciences Center, London, Ontario, Canada, CA aut NATIONALLICENCE 700 1- Cherian M. G. Departments of Pathology and Pharmacology and Toxicology, The University of Western Ontario, London, Canada, CA aut NATIONALLICENCE 700 1- Chakrabarti S. Department of Pathology, Medical Science Building, The University of Western Ontario, London, N6A 5C1, Canada e-mail: schakrab@julian.uwo.ca Fax: +1 519 661 3370, CA aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer