caa a22 4500 475742966 CHVBK 20180406123505.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s002400050142 doi (NATIONALLICENCE)springer-10.1007/s002400050142 Differentially expressed mRNAs in androgen-independent but not androgen-dependent Shionogi carcinoma [Elektronische Daten] [T. Takeuchi, H. Nishimatsu, T. Ueki, T. Kajiwara, H. Fukuhara, T. Ishida, N. Moriyama, T. Kitamura] Recently, a new and highly effective method termed suppressive subtractive hybridization (SSH) has been introduced to clone differentially expressed mRNAs. Genes expressed in androgen-independent but not in androgen-dependent tumors, and vice versa, are obviously significant to delineate the mechanisms of androgen dependency/independency of these tumors. Mouse mammary cancer (Shionogi carcinoma-115) has been extensively used to analyze the mechanism of androgen-dependent cancer growth. Methods: We cloned androgen-independent and androgen-dependent Shionogi carcinoma-115 specific mRNAs by the SSH method. Cloned sequences were compared with known sequences using NCBI BLAST across the Internet. Two clones were positive for cDNA insert when androgen-independent cDNA was used as tester cDNA, while no clones were positive using the androgen-dependent tester cDNA. One of the former was mouse protein kinase C beta-II while the other was a new DNA sequence. Mouse protein kinase C beta-II mRNA and the new mRNA were shown to be differentially expressed by RT-PCR analysis in androgen-independent but not androgen-dependent Shionogi carcinoma. Two mRNA species differentially expressed in androgen-independent but not androgen-dependent Shionogi carcinoma were cloned by the SSH method. The significance of these mRNAs for androgen-dependency/independency of Shionogi carcinoma should be explained in future studies. Springer-Verlag Berlin Heidelberg, 2000 Key words Cloning nationallicence cDNA nationallicence Androgen nationallicence Tumor nationallicence Takeuchi T. Department of Urology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan e-mail: TAKEUCHI-URO@h.u-tokyo.ac.jp Tel.: +81 3 5800 8662; Fax: +81 3 5800 8917, JP aut Nishimatsu H. Department of Urology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan e-mail: TAKEUCHI-URO@h.u-tokyo.ac.jp Tel.: +81 3 5800 8662; Fax: +81 3 5800 8917, JP aut Ueki T. Department of Urology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan e-mail: TAKEUCHI-URO@h.u-tokyo.ac.jp Tel.: +81 3 5800 8662; Fax: +81 3 5800 8917, JP aut Kajiwara T. Department of Urology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan e-mail: TAKEUCHI-URO@h.u-tokyo.ac.jp Tel.: +81 3 5800 8662; Fax: +81 3 5800 8917, JP aut Fukuhara H. Department of Urology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan e-mail: TAKEUCHI-URO@h.u-tokyo.ac.jp Tel.: +81 3 5800 8662; Fax: +81 3 5800 8917, JP aut Ishida T. Department of Urology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan e-mail: TAKEUCHI-URO@h.u-tokyo.ac.jp Tel.: +81 3 5800 8662; Fax: +81 3 5800 8917, JP aut Moriyama N. Department of Urology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan e-mail: TAKEUCHI-URO@h.u-tokyo.ac.jp Tel.: +81 3 5800 8662; Fax: +81 3 5800 8917, JP aut Kitamura T. Department of Urology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan e-mail: TAKEUCHI-URO@h.u-tokyo.ac.jp Tel.: +81 3 5800 8662; Fax: +81 3 5800 8917, JP aut https://doi.org/10.1007/s002400050142 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002400050142 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takeuchi T. Department of Urology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan e-mail: TAKEUCHI-URO@h.u-tokyo.ac.jp Tel.: +81 3 5800 8662; Fax: +81 3 5800 8917, JP aut NATIONALLICENCE 700 1- Nishimatsu H. Department of Urology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan e-mail: TAKEUCHI-URO@h.u-tokyo.ac.jp Tel.: +81 3 5800 8662; Fax: +81 3 5800 8917, JP aut NATIONALLICENCE 700 1- Ueki T. Department of Urology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan e-mail: TAKEUCHI-URO@h.u-tokyo.ac.jp Tel.: +81 3 5800 8662; Fax: +81 3 5800 8917, JP aut NATIONALLICENCE 700 1- Kajiwara T. Department of Urology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan e-mail: TAKEUCHI-URO@h.u-tokyo.ac.jp Tel.: +81 3 5800 8662; Fax: +81 3 5800 8917, JP aut NATIONALLICENCE 700 1- Fukuhara H. Department of Urology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan e-mail: TAKEUCHI-URO@h.u-tokyo.ac.jp Tel.: +81 3 5800 8662; Fax: +81 3 5800 8917, JP aut NATIONALLICENCE 700 1- Ishida T. Department of Urology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan e-mail: TAKEUCHI-URO@h.u-tokyo.ac.jp Tel.: +81 3 5800 8662; Fax: +81 3 5800 8917, JP aut NATIONALLICENCE 700 1- Moriyama N. Department of Urology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan e-mail: TAKEUCHI-URO@h.u-tokyo.ac.jp Tel.: +81 3 5800 8662; Fax: +81 3 5800 8917, JP aut NATIONALLICENCE 700 1- Kitamura T. Department of Urology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan e-mail: TAKEUCHI-URO@h.u-tokyo.ac.jp Tel.: +81 3 5800 8662; Fax: +81 3 5800 8917, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer