caa a22 4500 475743032 CHVBK 20180406123505.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s002400050141 doi (NATIONALLICENCE)springer-10.1007/s002400050141 Systemic treatment with epidermal growth factor but not insulin-like growth factor I decreases the involution of the prostate in castrated rats [Elektronische Daten] [Niels Tørring, Lars Vinter-Jensen, Flemming Brandt Sørensen, Allan Flyvbjerg, Ebba Nexø] Epidermal growth factor (EGF) and insulin-like growth factor I (IGF-I) are strong inducers of proliferation to prostate cells cultured in serum-free medium. Accordingly we wanted to study the growth of the prostate gland in castrated rats after treatment with EGF, IGF-I and testosterone. Castrated Wistar rats were treated with growth factors (EGF 35 μg/rat per day; IGF-I 350 μg/rat per day) or testosterone (2 mg/rat per day) for 3 days either immediately after or 10 days after castration. Prostate tissue was examined by stereological and immunohistochemical techniques and by enzyme-linked immunosorbent assay (ELISA). Treatment with EGF inhibited the involution of the prostate (P < 0.05), whereas treatment with IGF-I did not affect the prostate involution as compared to castrated controls. EGF treatment significantly increased the endogenous rat EGF in the ventral prostate, but cellular proliferation was not affected. Testosterone treatment increased the weight of the prostate, by increase of all tissue components of the prostate, and significantly increased cellular proliferation. Systemic administration of EGF but not IGF-I decreased the involution of the rat prostate induced by castration. Compared with testosterone, the effects of EGF treatment on the prostate involution were moderate, and the effects of EGF were not related to cellular proliferation. Springer-Verlag Berlin Heidelberg, 2000 Key words Castration nationallicence Epidermal growth factor nationallicence Insulin-like growth factor I nationallicence Prostate nationallicence Testosterone nationallicence Rat nationallicence Tørring Niels Department of Clinical Biochemistry-KH, Aarhus University Hospital, Nørrebrogade 44, DK-8000 Aarhus C, Denmark e-mail: ntorring@hotmail.com Tel.: +45-8949-3082; Fax: +45-8949-3060, DK aut Vinter-Jensen Lars Department of Clinical Biochemistry-KH, Aarhus University Hospital, Nørrebrogade 44, DK-8000 Aarhus C, Denmark e-mail: ntorring@hotmail.com Tel.: +45-8949-3082; Fax: +45-8949-3060, DK aut Brandt Sørensen Flemming Department of Pathology-AAS, Aarhus University Hospital, Denmark, DK aut Flyvbjerg Allan Medical Research Laboratory M (Diabetes and Endocrinology), Aarhus University Hospital, Denmark, DK aut Nexø Ebba Department of Clinical Biochemistry-KH, Aarhus University Hospital, Nørrebrogade 44, DK-8000 Aarhus C, Denmark e-mail: ntorring@hotmail.com Tel.: +45-8949-3082; Fax: +45-8949-3060, DK aut https://doi.org/10.1007/s002400050141 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s002400050141 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tørring Niels Department of Clinical Biochemistry-KH, Aarhus University Hospital, Nørrebrogade 44, DK-8000 Aarhus C, Denmark e-mail: ntorring@hotmail.com Tel.: +45-8949-3082; Fax: +45-8949-3060, DK aut NATIONALLICENCE 700 1- Vinter-Jensen Lars Department of Clinical Biochemistry-KH, Aarhus University Hospital, Nørrebrogade 44, DK-8000 Aarhus C, Denmark e-mail: ntorring@hotmail.com Tel.: +45-8949-3082; Fax: +45-8949-3060, DK aut NATIONALLICENCE 700 1- Brandt Sørensen Flemming Department of Pathology-AAS, Aarhus University Hospital, Denmark, DK aut NATIONALLICENCE 700 1- Flyvbjerg Allan Medical Research Laboratory M (Diabetes and Endocrinology), Aarhus University Hospital, Denmark, DK aut NATIONALLICENCE 700 1- Nexø Ebba Department of Clinical Biochemistry-KH, Aarhus University Hospital, Nørrebrogade 44, DK-8000 Aarhus C, Denmark e-mail: ntorring@hotmail.com Tel.: +45-8949-3082; Fax: +45-8949-3060, DK aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer