caa a22 4500 475743946 CHVBK 20180406123507.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1023/A:1026513528654 doi (NATIONALLICENCE)springer-10.1023/A:1026513528654 Isolation, Purification, and Characterization of Glutathione S-Transferase from Oat (Avena sativa) Seedlings [Elektronische Daten] [Vinu Zachariah, Nancy Walsh-Sayles, Bal Singh] Glutathione S-transferase (GST) from oat seedlings was purified by ammonium sulfate precipitation and glutathione (GSH) affinity chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed the presence of two major protein subunits with molecular masses of 29 and 31 kDa, respectively. Isoelectric focusing revealed a major band with pI of 3.43 and a minor band with pI of 7.42. Kinetic analysis with respect to 1-chloro-2,4-dinitrobenzene (CDNB) as substrate revealed a K m of 1.18 mM and V max of 0.94 μmol/min and a specific activity of 17.96 μmol/min/mg. Inhibition studies indicated that oat GST is strongly inhibited by chlorophyllin, hemin, and anthocyanin and only weakly by bilirubin and biliverdin. Plenum Publishing Corporation, 2000 Enzyme nationallicence glutathione S-transferase nationallicence oat nationallicence enzyme purification nationallicence tetrapyrroles nationallicence Zachariah Vinu Department of Chemistry and Biochemistry, and Center for Marine Science and Technology, University of Massachusetts Dartmouth, 02747, Dartmouth, Massachusetts aut Walsh-Sayles Nancy Department of Chemistry and Biochemistry, and Center for Marine Science and Technology, University of Massachusetts Dartmouth, 02747, Dartmouth, Massachusetts aut Singh Bal Department of Chemistry and Biochemistry, and Center for Marine Science and Technology, University of Massachusetts Dartmouth, 02747, Dartmouth, Massachusetts aut Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/6(2000-08-01), 425-430 0277-8033 19:6<425 2000 19 10930 https://doi.org/10.1023/A:1026513528654 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1026513528654 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zachariah Vinu Department of Chemistry and Biochemistry, and Center for Marine Science and Technology, University of Massachusetts Dartmouth, 02747, Dartmouth, Massachusetts aut NATIONALLICENCE 700 1- Walsh-Sayles Nancy Department of Chemistry and Biochemistry, and Center for Marine Science and Technology, University of Massachusetts Dartmouth, 02747, Dartmouth, Massachusetts aut NATIONALLICENCE 700 1- Singh Bal Department of Chemistry and Biochemistry, and Center for Marine Science and Technology, University of Massachusetts Dartmouth, 02747, Dartmouth, Massachusetts aut NATIONALLICENCE 773 0- Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/6(2000-08-01), 425-430 0277-8033 19:6<425 2000 19 10930 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer