caa a22 4500 475743954 CHVBK 20180406123507.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1023/A:1026513818176 doi (NATIONALLICENCE)springer-10.1023/A:1026513818176 Temperature, pH, and Solvent Isotope Effects on Cytochrome c Peroxidase Mutant N82A Studied by Proton NMR [Elektronische Daten] [James Satterlee, Jennifer Teske, James Erman, J. Mauro, Thomas Poulos] The mutant of baker's yeast cytochrome c peroxidase-CN with Ala82 in place of Asn82, [N82A]CcPCN, exhibits a complex solution behavior featuring dynamic interconversion among three enzyme forms that so far have only been detected by NMR spectroscopy. Proton NMR studies of [N82A]CcPCN reveal resonances from each of the three enzyme forms and show that the interconversion among forms is controlled by the pH, temperature, and isotope composition (H2O vs. D2O) of the buffer solution. No evidence for a key hydrogen bond between His52 and heme-coordinated cyanide is found in any of the enzyme forms, indicating that disruption of the extensive distal hydrogen bonding network is the source of this phenomenon. Plenum Publishing Corporation, 2000 Cytochrome c peroxidase nationallicence CcP nationallicence NMR nationallicence A82CcP mutant nationallicence solvent isotope effects nationallicence Satterlee James Department of Chemistry, Washington State University, 99164-4630, Pullman, Washington aut Teske Jennifer Department of Chemistry, Washington State University, 99164-4630, Pullman, Washington aut Erman James Department of Chemistry and Biochemistry, Northern Illinois University, 60115, DeKalb, Illinois aut Mauro J. Geo-Centers, Inc., 20744, Fort Washington, Maryland aut Poulos Thomas Department of Molecular Biology and Biochemistry, and Department of Physiology and Biophysics, University of California, 92717, Irvine, California aut Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/6(2000-08-01), 535-542 0277-8033 19:6<535 2000 19 10930 https://doi.org/10.1023/A:1026513818176 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1026513818176 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Satterlee James Department of Chemistry, Washington State University, 99164-4630, Pullman, Washington aut NATIONALLICENCE 700 1- Teske Jennifer Department of Chemistry, Washington State University, 99164-4630, Pullman, Washington aut NATIONALLICENCE 700 1- Erman James Department of Chemistry and Biochemistry, Northern Illinois University, 60115, DeKalb, Illinois aut NATIONALLICENCE 700 1- Mauro J. Geo-Centers, Inc., 20744, Fort Washington, Maryland aut NATIONALLICENCE 700 1- Poulos Thomas Department of Molecular Biology and Biochemistry, and Department of Physiology and Biophysics, University of California, 92717, Irvine, California aut NATIONALLICENCE 773 0- Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/6(2000-08-01), 535-542 0277-8033 19:6<535 2000 19 10930 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer