caa a22 4500 475744020 CHVBK 20180406123507.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1023/A:1026561701338 doi (NATIONALLICENCE)springer-10.1023/A:1026561701338 Conformational Studies of a Melittin—Inhibitor Complex [Elektronische Daten] [Yuen-Han Lam, Van Nguyen, Evgenia Fakaris, Frances Separovic] The conformation of a melittin—inhibitor complex was studied by solution NMR, solid-state NMR, and circular dichroism. In solution, binding was studied by titrating inhibitor against melittin in dimethyl sulfoxide, methanol, aqueous buffer, and dodecylphosphocholine micelles. The change in chemical shift of Trp19 resonances and the formation of a precipitate at 1:1 molar ratio indicated that the inhibitor was bound to melittin. Solid-state NMR also showed a change in chemical shift of two labeled carbons of melittin near Pro14 and a change in 1H T 1 relaxation times when complexed with inhibitor. Rotational resonance experiments of melittin labeled in the proline region indicated a change in conformation for melittin complexed with inhibitor. This observation was also supported by circular dichroism measurements, indicating a reduction in α-helical structure for increasing ratios of inhibitor bound to melittin. Plenum Publishing Corporation, 2000 Melittin nationallicence inhibitor nationallicence NMR nationallicence peptide nationallicence structure nationallicence Lam Yuen-Han School of Chemistry, University of Melbourne, 3010, Melbourne, VIC, Australia aut Nguyen Van School of Chemistry, University of Melbourne, 3010, Melbourne, VIC, Australia aut Fakaris Evgenia School of Chemistry, University of Melbourne, 3010, Melbourne, VIC, Australia aut Separovic Frances School of Chemistry, University of Melbourne, 3010, Melbourne, VIC, Australia aut Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/6(2000-08-01), 529-534 0277-8033 19:6<529 2000 19 10930 https://doi.org/10.1023/A:1026561701338 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1026561701338 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lam Yuen-Han School of Chemistry, University of Melbourne, 3010, Melbourne, VIC, Australia aut NATIONALLICENCE 700 1- Nguyen Van School of Chemistry, University of Melbourne, 3010, Melbourne, VIC, Australia aut NATIONALLICENCE 700 1- Fakaris Evgenia School of Chemistry, University of Melbourne, 3010, Melbourne, VIC, Australia aut NATIONALLICENCE 700 1- Separovic Frances School of Chemistry, University of Melbourne, 3010, Melbourne, VIC, Australia aut NATIONALLICENCE 773 0- Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/6(2000-08-01), 529-534 0277-8033 19:6<529 2000 19 10930 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer