caa a22 4500 475744039 CHVBK 20180406123507.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1023/A:1026541129563 doi (NATIONALLICENCE)springer-10.1023/A:1026541129563 Construction of a Ferritin Reactor: An Efficient Means for Trapping Various Heavy Metal Ions in Flowing Seawater [Elektronische Daten] [H. Huang, Q. Lin, Z. Lou] An apparatus consisting of two pumps, a mixer, a ferritin reactor, and a spectrophotometer was constructed to study the ability to trap various heavy metal ions (M2+) and the dynamics of a reconstituted ferritin reactor in flowing seawater. Reconstituted pig spleen ferritin (PSFr) is assembled from apo-protein shell to form a reconstituted iron core. The main components of the PSFr are its core, which contains an Fe2+:Pi stoichiometry of 6.0±0.5, reconstituted from pig spleen apoferritin (apo PSF), Fe2+, inorganic phosphate (Pi), and O2 (0.6 atm). The Fe3+—Pi clusters within the PSFr core exhibit resistance to salt ranging from 1% to 6% NaCl. The ferritin reactor consists of PSFr and an oscillating bag. Using the reactor, M2+ ions such as Cd2+, Zn2+, Co2+, and Mn2+ are directly trapped by the ferritin. We found a 1:2±0.2 stoichiometry of the trapped M2+ to the released iron as measured by chemical analysis or atomic absorption spectrometry; nontransient elements such as Na+, K+, Ca2+, etc., were scarcely trapped by the reactor. This study provides basic conditions for establishing a ferritin reactor and a convenient means for monitoring the pollution of heavy metal ions in seawater. Plenum Publishing Corporation, 2000 Ferritin reactor nationallicence heavy metal ions nationallicence trapping and storage nationallicence seawater nationallicence monitoring pollution nationallicence Huang H. Department of Biology, School of Life Sciences, Xiamen University, 361006, Xiamen, China aut Lin Q. The Research Center of Environment Science, Xiamen University, 361005, Xiamen, China aut Lou Z. Department of Biology, School of Life Sciences, Xiamen University, 361006, Xiamen, China aut Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/6(2000-08-01), 441-447 0277-8033 19:6<441 2000 19 10930 https://doi.org/10.1023/A:1026541129563 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1026541129563 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huang H. Department of Biology, School of Life Sciences, Xiamen University, 361006, Xiamen, China aut NATIONALLICENCE 700 1- Lin Q. The Research Center of Environment Science, Xiamen University, 361005, Xiamen, China aut NATIONALLICENCE 700 1- Lou Z. Department of Biology, School of Life Sciences, Xiamen University, 361006, Xiamen, China aut NATIONALLICENCE 773 0- Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/6(2000-08-01), 441-447 0277-8033 19:6<441 2000 19 10930 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer