caa a22 4500 475744160 CHVBK 20180406123508.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1023/A:1007099502179 doi (NATIONALLICENCE)springer-10.1023/A:1007099502179 α-to-β Structural Transformation of Ovalbumin: Heat and pH Effects [Elektronische Daten] [H. Hu, H. Du] Ovalbumin is an important member of the serpin superfamily without inhibitory activity. The heat- and pH-induced α-to-β structural transformations of ovalbumin were investigated by means of circular dichroism and binding of ANS and Congo red dyes. The native ovalbumin shows a mixture of α-helix and β-sheet, while both the heat and alkali treatments are able to transform the native protein into a predominance of β-sheet secondary structure. The free energy changes during transitions to the unfolded state are 5.19 kcal/mol from the native state and 4.00 kcal/mol from the heat-treated one. The binding abilities of the heat-treated and the alkali-treated forms to ANS and Congo red suggest that the altered forms exhibit hydrophobic exposure and intermolecular interaction. The results substantiate that the altered protein forms bearing increased β-sheet structures are prone to aggregation, which is implicated in the pathogenesis of some conformational diseases. Plenum Publishing Corporation, 2000 Ovalbumin nationallicence structural transformation nationallicence aggregation nationallicence heat nationallicence pH nationallicence Hu H. Shanghai Institute of Biochemistry, Chinese Academy of Sciences, 200031, Shanghai, China aut Du H. Shanghai Institute of Biochemistry, Chinese Academy of Sciences, 200031, Shanghai, China aut Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/3(2000-04-01), 177-183 0277-8033 19:3<177 2000 19 10930 https://doi.org/10.1023/A:1007099502179 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1007099502179 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hu H. Shanghai Institute of Biochemistry, Chinese Academy of Sciences, 200031, Shanghai, China aut NATIONALLICENCE 700 1- Du H. Shanghai Institute of Biochemistry, Chinese Academy of Sciences, 200031, Shanghai, China aut NATIONALLICENCE 773 0- Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/3(2000-04-01), 177-183 0277-8033 19:3<177 2000 19 10930 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer