caa a22 4500 475744217 CHVBK 20180406123508.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1023/A:1007007803996 doi (NATIONALLICENCE)springer-10.1023/A:1007007803996 Purification and Properties of a Nucleotide Pyrophosphatase from Lentil Seedlings [Elektronische Daten] [Rosaria Medda, Alessandra Padiglia, Anita Lorrai, Barbara Murgia, Alessandro Agrò, Massimo Castagnola, Giovanni Floris] A nucleotide pyrophosphatase (EC 3.6.1.9) was purified to homogeneity from lentil seedlings. The enzyme is a single polypeptide chain of 75 ± 2 kDa that exhibits hydrolytic activities toward pyrophosphate linkages of several substrates. Reduced and oxidized forms of NAD(P) were shown to be hydrolyzed to nicotinamide mononucleotide and AMP. Other dinucleotides such as FAD and dinucleoside oligophosphates were hydrolyzed as well, but with lower efficiency. Pyrophosphatase activity was increased in the presence of divalent cations such as Ca2+, Mg2+, and Mn2+, whereas Cu2+, Zn2+, and Ni2+ ions inhibited this activity. The active site in the enzyme was not defined, but histidine residue(s) seemed to be crucial for the enzymatic activity. Plenum Publishing Corporation, 2000 Lens esculenta nationallicence Leguminosae nationallicence nucleotide pyrophosphatase nationallicence nucleotides nationallicence Medda Rosaria Department of Sciences Applied to Biosystems, University of Cagliari, Città Universitaria, 09042, Monserrato (CA), Italy aut Padiglia Alessandra Department of Sciences Applied to Biosystems, University of Cagliari, Città Universitaria, 09042, Monserrato (CA), Italy aut Lorrai Anita Department of Sciences Applied to Biosystems, University of Cagliari, Città Universitaria, 09042, Monserrato (CA), Italy aut Murgia Barbara Department of Sciences Applied to Biosystems, University of Cagliari, Città Universitaria, 09042, Monserrato (CA), Italy aut Agrò Alessandro Department of Experimental Medicine, University of Rome "Tor Vergata”, Rome, Italy aut Castagnola Massimo Department of Sciences Applied to Biosystems, University of Cagliari, Città Universitaria, 09042, Monserrato (CA), Italy aut Floris Giovanni Department of Sciences Applied to Biosystems, University of Cagliari, Città Universitaria, 09042, Monserrato (CA), Italy aut Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/3(2000-04-01), 209-214 0277-8033 19:3<209 2000 19 10930 https://doi.org/10.1023/A:1007007803996 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1007007803996 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Medda Rosaria Department of Sciences Applied to Biosystems, University of Cagliari, Città Universitaria, 09042, Monserrato (CA), Italy aut NATIONALLICENCE 700 1- Padiglia Alessandra Department of Sciences Applied to Biosystems, University of Cagliari, Città Universitaria, 09042, Monserrato (CA), Italy aut NATIONALLICENCE 700 1- Lorrai Anita Department of Sciences Applied to Biosystems, University of Cagliari, Città Universitaria, 09042, Monserrato (CA), Italy aut NATIONALLICENCE 700 1- Murgia Barbara Department of Sciences Applied to Biosystems, University of Cagliari, Città Universitaria, 09042, Monserrato (CA), Italy aut NATIONALLICENCE 700 1- Agrò Alessandro Department of Experimental Medicine, University of Rome "Tor Vergata”, Rome, Italy aut NATIONALLICENCE 700 1- Castagnola Massimo Department of Sciences Applied to Biosystems, University of Cagliari, Città Universitaria, 09042, Monserrato (CA), Italy aut NATIONALLICENCE 700 1- Floris Giovanni Department of Sciences Applied to Biosystems, University of Cagliari, Città Universitaria, 09042, Monserrato (CA), Italy aut NATIONALLICENCE 773 0- Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/3(2000-04-01), 209-214 0277-8033 19:3<209 2000 19 10930 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer