caa a22 4500 475744276 CHVBK 20180406123508.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1023/A:1007095329303 doi (NATIONALLICENCE)springer-10.1023/A:1007095329303 The Two Slow Refolding Processes of Creatine Kinase Are Catalyzed by Cyclophilin [Elektronische Daten] [Guo-Chang Huang, Jun-Mei Zhou] A burst phase occurs in the refolding kinetics of guanidine-denatured creatine kinase due to formation of an intermediate within the mixing dead time, with further refolding to the native state after the burst phase along a path following biphasic kinetics. In the presence of cyclophilin, the refolding rates of the two slow processes are accelerated and the values are proportional to the cyclophilin concentration. The activity of cyclophilin in accelerating the slow refolding processes of creatine kinase is totally inhibited by cyclosporin A, indicating that the cis—trans isomerization of the peptidyl—prolyl bonds is involved in the two slow refolding processes. Plenum Publishing Corporation, 2000 Creatine kinase nationallicence cyclophilin nationallicence cyclosporin A nationallicence protein refolding nationallicence cis—trans isomerization nationallicence Huang Guo-Chang Institute of Biophysics, Academia Sinica, National Laboratory of Biomacromolecules, 100101, Beijing, China aut Zhou Jun-Mei Institute of Biophysics, Academia Sinica, National Laboratory of Biomacromolecules, 100101, Beijing, China aut Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/4(2000-05-01), 285-289 0277-8033 19:4<285 2000 19 10930 https://doi.org/10.1023/A:1007095329303 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1007095329303 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huang Guo-Chang Institute of Biophysics, Academia Sinica, National Laboratory of Biomacromolecules, 100101, Beijing, China aut NATIONALLICENCE 700 1- Zhou Jun-Mei Institute of Biophysics, Academia Sinica, National Laboratory of Biomacromolecules, 100101, Beijing, China aut NATIONALLICENCE 773 0- Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/4(2000-05-01), 285-289 0277-8033 19:4<285 2000 19 10930 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer