caa a22 4500 475744381 CHVBK 20180406123509.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1023/A:1007192017291 doi (NATIONALLICENCE)springer-10.1023/A:1007192017291 Intrinsic Tryptophan Fluorescence of Human Serum Proteins and Related Conformational Changes [Elektronische Daten] [Shela Gorinstein, Ivan Goshev, Snejana Moncheva, Marina Zemser, Moshe Weisz, Abraham Caspi, Imanuel Libman, Henry Lerner, Simon Trakhtenberg, Olga Martín-Belloso] The unfolding of human serum proteins (HSP) was studied by measuring the intrinsic fluorescence intensity at a wavelength of excitation corresponding to tryptophan's or typosine's fluorescence and surface hydrophobicity. The maxima emission wavelengths (λmax) of human serum albumin (HSA) and human serum globulin (HSG) before beer consumption (BC) were 336.0 and 337.0 nm and after BC shifted to 335.0 and 334.0 nm, respectively. The surface hydrophobicity slightly increased after BC. In a solution of 8 M urea the λmax of BSA shifted to 346.4 and that of BSG to 342.5 nm. In contrast, in the same solution but after BC the λmax positions of HSA and HSG shifted to 355.9 and 357.7 nm, respectively. A decrease in fluorescence intensity, a shift in the maximum of emission, and an increase in surface hydrophobicity which reflected unfolding of proteins were observed. Here we provide evidence that the loosening of the HSP structure takes place primarily in various concentrations of urea before and after beer consumption. Differences in the fluorescence behavior of the proteins are attributed to disruption of the structure of proteins by denaturants as well as by the change in their compactability as a result of ethanol consumption. Plenum Publishing Corporation, 2000 Human serum proteins nationallicence fluorescence nationallicence ethanol nationallicence denaturation nationallicence Gorinstein Shela Department of Medicinal Chemistry, School of Pharmacy, The Hebrew University—Hadassah Medical School, P.O.B. 12065, 91120, Jerusalem, Israel aut Goshev Ivan Institute of Organic Chemistry with Center of Phytochemistry, Bulgarian Academy of Sciences, Sofia, Bulgaria aut Moncheva Snejana Department of Biology, Institute of Oceanology, Bulgarian Academy of Sciences, Varna, Bulgaria aut Zemser Marina Institute of Standards, Medical Laboratory, Ministry of Health, Jerusalem, Israel aut Weisz Moshe Department of Medicinal Chemistry, School of Pharmacy, The Hebrew University—Hadassah Medical School, P.O.B. 12065, 91120, Jerusalem, Israel aut Caspi Abraham Kaplan Medical Center, Rehovot, Israel aut Libman Imanuel Kaplan Medical Center, Rehovot, Israel aut Lerner Henry Kaplan Medical Center, Rehovot, Israel aut Trakhtenberg Simon Kaplan Medical Center, Rehovot, Israel aut Martín-Belloso Olga Food Technology Department, University of Lleida, Lleida, Spain aut Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/8(2000-11-01), 637-642 0277-8033 19:8<637 2000 19 10930 https://doi.org/10.1023/A:1007192017291 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1007192017291 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gorinstein Shela Department of Medicinal Chemistry, School of Pharmacy, The Hebrew University—Hadassah Medical School, P.O.B. 12065, 91120, Jerusalem, Israel aut NATIONALLICENCE 700 1- Goshev Ivan Institute of Organic Chemistry with Center of Phytochemistry, Bulgarian Academy of Sciences, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Moncheva Snejana Department of Biology, Institute of Oceanology, Bulgarian Academy of Sciences, Varna, Bulgaria aut NATIONALLICENCE 700 1- Zemser Marina Institute of Standards, Medical Laboratory, Ministry of Health, Jerusalem, Israel aut NATIONALLICENCE 700 1- Weisz Moshe Department of Medicinal Chemistry, School of Pharmacy, The Hebrew University—Hadassah Medical School, P.O.B. 12065, 91120, Jerusalem, Israel aut NATIONALLICENCE 700 1- Caspi Abraham Kaplan Medical Center, Rehovot, Israel aut NATIONALLICENCE 700 1- Libman Imanuel Kaplan Medical Center, Rehovot, Israel aut NATIONALLICENCE 700 1- Lerner Henry Kaplan Medical Center, Rehovot, Israel aut NATIONALLICENCE 700 1- Trakhtenberg Simon Kaplan Medical Center, Rehovot, Israel aut NATIONALLICENCE 700 1- Martín-Belloso Olga Food Technology Department, University of Lleida, Lleida, Spain aut NATIONALLICENCE 773 0- Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/8(2000-11-01), 637-642 0277-8033 19:8<637 2000 19 10930 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer