caa a22 4500 47574439X CHVBK 20180406123509.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1023/A:1007156503082 doi (NATIONALLICENCE)springer-10.1023/A:1007156503082 Purification, Characterization, and Relation to Bikunin of Rat Urinary Trypsin Inhibitors [Elektronische Daten] [Akie Kurata, Kiyoshige Ohi, Kenji Sato, Misao Tashiro] Two forms of urinary trypsin inhibitor (UTI-1 and UTI-2) were purified from pooled urine of normal male rats to apparent homogeneity by salting out, affinity chromatography, gel filtration, and reverse-phase HPLC. UTIs-1 and 2 were shown to be thermostable glycoproteins with the respective molecular weights of 22,000 and 18,000 estimated by SDS-PAGE. These inhibitors combined with bovine trypsin in a 1:1 molar ratio: the K d values were 2.5 × 10−10 and 2.3 × 10−10 M, respectively. Amino acid composition and sequence analysis indicated that UTI-1 corresponded to rat bikunin of which the amino acid sequence was deduced from a rat liver cDNA clone encoding α1-microglobulin [Lindqvist et al. (1992), Biochim. Biophys. Acta 1130, 63-67] except that the protein sequence seemed to lack C-terminal serine, and UTI-2 corresponded to UTI-1 lacking N-terminal 21 amino acid residues. Plenum Publishing Corporation, 2000 Bikunin nationallicence glycoprotein nationallicence trypsin nationallicence urinary trypsin inhibitor nationallicence Kurata Akie College of Human Ecology, Kyoto Koka Women's University, Nishikyogoku, Ukyo-ku, 615-0882, Kyoto, Japan aut Ohi Kiyoshige Toyobouseki Company, Katada, Ohtsu, 520-0243, Shiga, Japan aut Sato Kenji Faculty of Human Environment, Kyoto Prefectural University, Sakyo-ku, 606-5822, Kyoto, Japan aut Tashiro Misao School of Human Environmental Sciences and Interdisciplinary Research Institute for Biosciences, Mukogawa Women's University, Nishinomiya, 663-8558, Hyogo, Japan aut Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/8(2000-11-01), 693-698 0277-8033 19:8<693 2000 19 10930 https://doi.org/10.1023/A:1007156503082 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1007156503082 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kurata Akie College of Human Ecology, Kyoto Koka Women's University, Nishikyogoku, Ukyo-ku, 615-0882, Kyoto, Japan aut NATIONALLICENCE 700 1- Ohi Kiyoshige Toyobouseki Company, Katada, Ohtsu, 520-0243, Shiga, Japan aut NATIONALLICENCE 700 1- Sato Kenji Faculty of Human Environment, Kyoto Prefectural University, Sakyo-ku, 606-5822, Kyoto, Japan aut NATIONALLICENCE 700 1- Tashiro Misao School of Human Environmental Sciences and Interdisciplinary Research Institute for Biosciences, Mukogawa Women's University, Nishinomiya, 663-8558, Hyogo, Japan aut NATIONALLICENCE 773 0- Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/8(2000-11-01), 693-698 0277-8033 19:8<693 2000 19 10930 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer