caa a22 4500 475744446 CHVBK 20180406123509.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1023/A:1007164619921 doi (NATIONALLICENCE)springer-10.1023/A:1007164619921 Recombinant Derivatives of Clostridial Neurotoxins as Delivery Vehicles for Proteins and Small Organic Molecules [Elektronische Daten] [Marina Zdanovskaia, Georgyi Los, Alexey Zdanovsky] Clostridial neurotoxins are the most powerful toxins known. Nevertheless, derivatives of these toxins may find broad applications both in science and medicine because of their unique abilities to recognize neurons and deliver small and large molecules into them. In this paper we describe the construction of two types of such derivatives. Proteins belonging to the first class were designed to allow direct conjugation with one or few molecules of interest. Proteins belonging to the second class contain biotin residue and therefore could be easily connected to streptavidin loaded with multiple molecules of interest. Only C-terminal regions of neurotoxin heavy chains were incorporated in the structure of recombinant proteins. Nevertheless, recombinant proteins were found to be able to recognize specific neuronal receptors and target model molecules to rat synaptosomes and human neuroblastoma cells. Plenum Publishing Corporation, 2000 Expression nationallicence clostridial neurotoxins nationallicence targeting nationallicence neurons nationallicence Zdanovskaia Marina Promega Corporation, 2800 Woods Hollow Road, 53711-5399, Madison, Wisconsin aut Los Georgyi Promega Corporation, 2800 Woods Hollow Road, 53711-5399, Madison, Wisconsin aut Zdanovsky Alexey Promega Corporation, 2800 Woods Hollow Road, 53711-5399, Madison, Wisconsin aut Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/8(2000-11-01), 699-707 0277-8033 19:8<699 2000 19 10930 https://doi.org/10.1023/A:1007164619921 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1007164619921 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zdanovskaia Marina Promega Corporation, 2800 Woods Hollow Road, 53711-5399, Madison, Wisconsin aut NATIONALLICENCE 700 1- Los Georgyi Promega Corporation, 2800 Woods Hollow Road, 53711-5399, Madison, Wisconsin aut NATIONALLICENCE 700 1- Zdanovsky Alexey Promega Corporation, 2800 Woods Hollow Road, 53711-5399, Madison, Wisconsin aut NATIONALLICENCE 773 0- Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/8(2000-11-01), 699-707 0277-8033 19:8<699 2000 19 10930 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer