caa a22 4500 475744470 CHVBK 20180406123509.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1023/A:1026431329188 doi (NATIONALLICENCE)springer-10.1023/A:1026431329188 The Isolation and Properties of the Dimeric Subunit of Concanavalin A [Elektronische Daten] [John Pazur, Michael Perloff, Adam Frymoyer, Carolyn Jensen, Holly Micolochick, Andrea Mastro] Concanavalin A (Con A) was dissociated into dimeric and monomeric subunits by incubation at 37°C in acetate buffer of pH 3.8 containing 0.5% sodium dodecyl sulfate. The dimer was isolated in pure form by a density gradient ultracentrifugation method. Several properties of the dimer were determined including the formation of a precipitin with anti-Con A antibodies, the molecular weight, the lack of a binding site for glycogen, the lack of mitogenic activity for spleen lymphocytes, and the lack of inhibition by α-methyl d-glucoside. The latter findings differ from results reported by other investigators. Plenum Publishing Corporation, 2000 Concanavalin A (Con A) nationallicence dimeric subunit nationallicence biological properties nationallicence Pazur John Department of Biochemistry and Molecular Biology, Pennsylvania State University, 16802, University Park, Pennsylvania aut Perloff Michael Department of Biochemistry and Molecular Biology, Pennsylvania State University, 16802, University Park, Pennsylvania aut Frymoyer Adam Department of Biochemistry and Molecular Biology, Pennsylvania State University, 16802, University Park, Pennsylvania aut Jensen Carolyn Department of Biology, Pennsylvania State University, 16802, University Park, Pennsylvania aut Micolochick Holly Department of Biochemistry and Molecular Biology, Pennsylvania State University, 16802, University Park, Pennsylvania aut Mastro Andrea Department of Biochemistry and Molecular Biology, Pennsylvania State University, 16802, University Park, Pennsylvania aut Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/5(2000-07-01), 353-359 0277-8033 19:5<353 2000 19 10930 https://doi.org/10.1023/A:1026431329188 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1026431329188 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pazur John Department of Biochemistry and Molecular Biology, Pennsylvania State University, 16802, University Park, Pennsylvania aut NATIONALLICENCE 700 1- Perloff Michael Department of Biochemistry and Molecular Biology, Pennsylvania State University, 16802, University Park, Pennsylvania aut NATIONALLICENCE 700 1- Frymoyer Adam Department of Biochemistry and Molecular Biology, Pennsylvania State University, 16802, University Park, Pennsylvania aut NATIONALLICENCE 700 1- Jensen Carolyn Department of Biology, Pennsylvania State University, 16802, University Park, Pennsylvania aut NATIONALLICENCE 700 1- Micolochick Holly Department of Biochemistry and Molecular Biology, Pennsylvania State University, 16802, University Park, Pennsylvania aut NATIONALLICENCE 700 1- Mastro Andrea Department of Biochemistry and Molecular Biology, Pennsylvania State University, 16802, University Park, Pennsylvania aut NATIONALLICENCE 773 0- Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/5(2000-07-01), 353-359 0277-8033 19:5<353 2000 19 10930 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer