caa a22 4500 475744535 CHVBK 20180406123509.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1023/A:1026446219097 doi (NATIONALLICENCE)springer-10.1023/A:1026446219097 Chemical Modification of a Catalytic Antibody That Accelerates the Hydrolysis of Carbonate Esters [Elektronische Daten] [Haruo Suzuki, Yuhki Higashi, Nobutaka Naitoh, Chiharu Yamamoto, Sayaka Nakamura, Yasuko Kawamura-Konishi] Catalytic antibody, 4A1, catalyzes the hydrolysis of p-nitrophenyl alkyl carbonate. To determine the amino acid residues related to the catalytic activity of the antibody, we studied the effect of Tyr-, Trp-, and Lys-selective reagents on the catalytic activity and determined the amino acid sequences around the modified amino acid residues. We found that the Tyr-selective reagent is the most effective one and the modification of one Tyr residue results in the complete loss of the catalytic activity. The modified Tyr residue is identified to be Tyr-32 in the CDR-1 of the L chain. Plenum Publishing Corporation, 2000 Catalytic antibody nationallicence abzyme nationallicence sequence determination nationallicence Tyr residue nationallicence chemical modification nationallicence Suzuki Haruo Department of Biosciences, School of Science, Kitasato University, 1-15-1 Kitasato, Sagamihara, 228-8555, Kanagawa, Japan aut Higashi Yuhki Department of Biosciences, School of Science, Kitasato University, 1-15-1 Kitasato, Sagamihara, 228-8555, Kanagawa, Japan aut Naitoh Nobutaka Department of Biosciences, School of Science, Kitasato University, 1-15-1 Kitasato, Sagamihara, 228-8555, Kanagawa, Japan aut Yamamoto Chiharu Department of Biosciences, School of Science, Kitasato University, 1-15-1 Kitasato, Sagamihara, 228-8555, Kanagawa, Japan aut Nakamura Sayaka Department of Biosciences, School of Science, Kitasato University, 1-15-1 Kitasato, Sagamihara, 228-8555, Kanagawa, Japan aut Kawamura-Konishi Yasuko Department of Biosciences, School of Science, Kitasato University, 1-15-1 Kitasato, Sagamihara, 228-8555, Kanagawa, Japan aut Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/5(2000-07-01), 419-424 0277-8033 19:5<419 2000 19 10930 https://doi.org/10.1023/A:1026446219097 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1026446219097 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Suzuki Haruo Department of Biosciences, School of Science, Kitasato University, 1-15-1 Kitasato, Sagamihara, 228-8555, Kanagawa, Japan aut NATIONALLICENCE 700 1- Higashi Yuhki Department of Biosciences, School of Science, Kitasato University, 1-15-1 Kitasato, Sagamihara, 228-8555, Kanagawa, Japan aut NATIONALLICENCE 700 1- Naitoh Nobutaka Department of Biosciences, School of Science, Kitasato University, 1-15-1 Kitasato, Sagamihara, 228-8555, Kanagawa, Japan aut NATIONALLICENCE 700 1- Yamamoto Chiharu Department of Biosciences, School of Science, Kitasato University, 1-15-1 Kitasato, Sagamihara, 228-8555, Kanagawa, Japan aut NATIONALLICENCE 700 1- Nakamura Sayaka Department of Biosciences, School of Science, Kitasato University, 1-15-1 Kitasato, Sagamihara, 228-8555, Kanagawa, Japan aut NATIONALLICENCE 700 1- Kawamura-Konishi Yasuko Department of Biosciences, School of Science, Kitasato University, 1-15-1 Kitasato, Sagamihara, 228-8555, Kanagawa, Japan aut NATIONALLICENCE 773 0- Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/5(2000-07-01), 419-424 0277-8033 19:5<419 2000 19 10930 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer