caa a22 4500 475744578 CHVBK 20180406123509.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1023/A:1007078415595 doi (NATIONALLICENCE)springer-10.1023/A:1007078415595 New Genetic Variants Identified in Donkey's Milk Whey Proteins [Elektronische Daten] [Maryse Herrouin, Daniel Mollé, Jacques Fauquant, François Ballestra, Jean-Louis Maubois, Joëlle Léonil] Novel genetic variants for donkey milk lysozyme and β-lactoglobulins I and II have been identified by the combined use of peptide mass mapping and sequencing by tandem mass spectrometry in association with database searching. The novel donkey lysozyme variant designated as lysozyme B (Mr 14,631 Da) differed in three amino acid exchanges, N49 → D, Y52 → S, and S61 → N, from the previously published sequence. Three novel genetic variants for donkey β-lactoglobulins were identified. One of them is a type β-lactoglobulin I with three amino acid exchanges at E36 → S, S97 → T, and V150 → I (β-lactoglobulin I B, Mr 18,510 Da). The two others are type β-lactoglobulins II with two amino acid exchanges at C110 → P and M118 → T (β-lactoglobulin II B, Mr 18,227 Da) and with three amino acid exchanges at D96 → E, C110 → P, and M118 → T (β-lactoglobulin II C, Mr 18,241 Da). All these primary structures are closely related to those of homologous proteins in horse milk (percent identity >96%). Plenum Publishing Corporation, 2000 Mass spectrometry nationallicence protein sequence nationallicence whey protein nationallicence donkey milk nationallicence Herrouin Maryse EURL François Ballestra, SAINT-RAPHAËL, France aut Mollé Daniel INRA, Laboratoire de Recherche en Technologie Laitière, 35042, Rennes Cedex, France aut Fauquant Jacques INRA, Laboratoire de Recherche en Technologie Laitière, 35042, Rennes Cedex, France aut Ballestra François EURL François Ballestra, SAINT-RAPHAËL, France aut Maubois Jean-Louis INRA, Laboratoire de Recherche en Technologie Laitière, 35042, Rennes Cedex, France aut Léonil Joëlle INRA, Laboratoire de Recherche en Technologie Laitière, 35042, Rennes Cedex, France aut Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/2(2000-02-01), 105-116 0277-8033 19:2<105 2000 19 10930 https://doi.org/10.1023/A:1007078415595 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1007078415595 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Herrouin Maryse EURL François Ballestra, SAINT-RAPHAËL, France aut NATIONALLICENCE 700 1- Mollé Daniel INRA, Laboratoire de Recherche en Technologie Laitière, 35042, Rennes Cedex, France aut NATIONALLICENCE 700 1- Fauquant Jacques INRA, Laboratoire de Recherche en Technologie Laitière, 35042, Rennes Cedex, France aut NATIONALLICENCE 700 1- Ballestra François EURL François Ballestra, SAINT-RAPHAËL, France aut NATIONALLICENCE 700 1- Maubois Jean-Louis INRA, Laboratoire de Recherche en Technologie Laitière, 35042, Rennes Cedex, France aut NATIONALLICENCE 700 1- Léonil Joëlle INRA, Laboratoire de Recherche en Technologie Laitière, 35042, Rennes Cedex, France aut NATIONALLICENCE 773 0- Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/2(2000-02-01), 105-116 0277-8033 19:2<105 2000 19 10930 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer