caa a22 4500 475744616 CHVBK 20180406123509.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1023/A:1007042902391 doi (NATIONALLICENCE)springer-10.1023/A:1007042902391 Mannose-Specific Isolectins with Different Hemagglutinating Potencies Isolated from Chinese Daffodil (Narcissus tazetta var. chinensis) Leaves [Elektronische Daten] [Linda Ooi, T. Ng, Samuel Sun, Vincent Ooi] Three mannose-specific lectins exhibiting considerable similarities in NH2-terminal amino acid sequence were isolated from leaves of the Chinese daffodil Narcissus tazetta (Family Amaryllidaceae). The purification protocol involved extraction with an aqueous buffer, anion exchange chromatography on DEAE-cellulose using stepwise elution with increasing salt concentrations, affinity chromatography on mannose-agarose, and FPLC-gel filtration on Superose 12. From the peak unadsorbed on DEAE-cellulose, and two peaks adsorbed on the ion exchanger and eluted respectively with 0.2 M Tris-HCl buffer and 0.5 M NaCl, were prepared fractions which yielded isolectins 1, 2, and 3 after adsorption on mannose-agarose and FPLC-gel filtraton. All three isolectins were homodimers with a molecular weight of 26 kDa. The lectin unadsorbed on DEAE-cellulose had the lowest, while the most strongly adsorbed lectin had the highest hemagglutinating activity. Plenum Publishing Corporation, 2000 Daffodil nationallicence mannose-binding lectin nationallicence Ooi Linda Department of Biochemistry, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong aut Ng T. Department of Biochemistry, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong aut Sun Samuel Department of Biology, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong aut Ooi Vincent Department of Biology, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong aut Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/2(2000-02-01), 163-168 0277-8033 19:2<163 2000 19 10930 https://doi.org/10.1023/A:1007042902391 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1007042902391 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ooi Linda Department of Biochemistry, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong aut NATIONALLICENCE 700 1- Ng T. Department of Biochemistry, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong aut NATIONALLICENCE 700 1- Sun Samuel Department of Biology, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong aut NATIONALLICENCE 700 1- Ooi Vincent Department of Biology, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong aut NATIONALLICENCE 773 0- Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/2(2000-02-01), 163-168 0277-8033 19:2<163 2000 19 10930 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer