caa a22 4500 475744632 CHVBK 20180406123509.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1023/A:1007074314686 doi (NATIONALLICENCE)springer-10.1023/A:1007074314686 Influence of Neutral Salts on the Hydrothermal Stability of Acid-Soluble Collagen [Elektronische Daten] [Eleanor Brown, Harold Farrell Jr., Renee Wildermuth] The thermal stability of acid-soluble collagens was studied by circular dichroism (CD) spectroscopy. Adult bovine dermal collagen (BDC), rat-tail tendon collagen (RTC), and calf skin collagen (CSC) were compared. Despite some variability in amino acid composition and apparent molecular weight, the CD spectra for helical and unordered collagen structures were essentially the same for all the sources. The melting of these collagens occurs as a two-stage process characterized by a pretransition (T p) followed by complete denaturation (T d). The characteristic temperatures vary with the source of the collagen; for mature collagens (BDC, RTC) T p = 30°C and T d = 36deg;C, and for CSC T p = 34°C and T d = 40°C. Neutral salts, NaCl or KCl, at low concentrations (0.02-0.2 M) appear to bind to the collagens and shift the thermal transitions of these collagens to lower temperatures. Plenum Publishing Corporation, 2000 Soluble collagen nationallicence thermal stability nationallicence NaCl nationallicence KCl nationallicence Brown Eleanor Eastern Regional Research Center, United States Department of Agriculture, Agricultural Research Service, 19038, Wyndmoor, Pennsylvania aut Farrell Jr. Harold Eastern Regional Research Center, United States Department of Agriculture, Agricultural Research Service, 19038, Wyndmoor, Pennsylvania aut Wildermuth Renee Eastern Regional Research Center, United States Department of Agriculture, Agricultural Research Service, 19038, Wyndmoor, Pennsylvania aut Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/2(2000-02-01), 85-92 0277-8033 19:2<85 2000 19 10930 https://doi.org/10.1023/A:1007074314686 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1007074314686 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Brown Eleanor Eastern Regional Research Center, United States Department of Agriculture, Agricultural Research Service, 19038, Wyndmoor, Pennsylvania aut NATIONALLICENCE 700 1- Farrell Jr Harold Eastern Regional Research Center, United States Department of Agriculture, Agricultural Research Service, 19038, Wyndmoor, Pennsylvania aut NATIONALLICENCE 700 1- Wildermuth Renee Eastern Regional Research Center, United States Department of Agriculture, Agricultural Research Service, 19038, Wyndmoor, Pennsylvania aut NATIONALLICENCE 773 0- Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/2(2000-02-01), 85-92 0277-8033 19:2<85 2000 19 10930 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer