caa a22 4500 475744667 CHVBK 20180406123509.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1023/A:1007038801482 doi (NATIONALLICENCE)springer-10.1023/A:1007038801482 Kinetics of c-Phycocyanin Reaction with Hypochlorite [Elektronische Daten] [Cheyla Romay, Ricardo Gonzalez, Marta Pizarro, Eduardo Lissi] Hydrochlorous acid bleaches c-phycocyanin visible absorbance with a second-order rate constant (pH 7.4) of 1.3 × 103 M−1 s−1. In excess of protein, ca, 0.16 bilin moieties are disrupted by each reacted HOCl molecule. This indicates that the main reaction takes place at the apoprotein level, with a total rate constant (in monomeric units concentration) of 2.5 × 104 M−1 s−1. This rate constant is too low to provide protection to other biomolecules under physiological conditions. The reported antiinflammatory properties of phycocyanin are not then related to the removal of HOCl. On the other hand, the rather slow reaction rate with HOCl could be beneficial to its role as antiinflammatory agent since it will allow the protein to maintain its integrity at the inflammation locus. Plenum Publishing Corporation, 2000 c-Phycocyanin nationallicence hypopchlorite nationallicence bilin modification nationallicence reaction rate nationallicence Romay Cheyla Pharmacology Department, National Center for Scientific Research, CNIC, Havana, Cuba aut Gonzalez Ricardo Pharmacology Department, National Center for Scientific Research, CNIC, Havana, Cuba aut Pizarro Marta Chemistry Department, Faculty of Chemistry and Biology, University of Santiago, Santiago, Chile aut Lissi Eduardo Chemistry Department, Faculty of Chemistry and Biology, University of Santiago, Santiago, Chile aut Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/2(2000-02-01), 151-155 0277-8033 19:2<151 2000 19 10930 https://doi.org/10.1023/A:1007038801482 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1007038801482 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Romay Cheyla Pharmacology Department, National Center for Scientific Research, CNIC, Havana, Cuba aut NATIONALLICENCE 700 1- Gonzalez Ricardo Pharmacology Department, National Center for Scientific Research, CNIC, Havana, Cuba aut NATIONALLICENCE 700 1- Pizarro Marta Chemistry Department, Faculty of Chemistry and Biology, University of Santiago, Santiago, Chile aut NATIONALLICENCE 700 1- Lissi Eduardo Chemistry Department, Faculty of Chemistry and Biology, University of Santiago, Santiago, Chile aut NATIONALLICENCE 773 0- Journal of Protein Chemistry Kluwer Academic Publishers-Plenum Publishers 19/2(2000-02-01), 151-155 0277-8033 19:2<151 2000 19 10930 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer