caa a22 4500 475744713 CHVBK 20180406123509.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1023/A:1005609319445 doi (NATIONALLICENCE)springer-10.1023/A:1005609319445 The length dependency of calcium activated contractions in the femoral artery smooth muscle studied with different methods of skinning [Elektronische Daten] [B. Van Heijst, T. Blangé, H. Jongsma, E. De Beer] The relationship between the calcium concentration and the isometric tension obtained with different techniques of skinning provides information on the biochemical events of contraction in vascular smooth muscle. Muscle preparations of the rabbit femoral artery were skinned with triton X-100, saponin, β-escin and α-toxin and the relationship between the calcium concentration and isometric tension was determined at different preparation lengths. We determined the calcium sensitivity as a function of muscle length with different techniques of skinning. At a pCa of 6.0, triton X-100 skinned smooth muscle of the femoral artery generated 50% of the maximal tension. In α-toxin skinned preparations, this calcium sensitivity was shifted to a pCa of 5.6. The sensitivity of the saponin and β-escin skinned preparations were in between those of the triton X-100 and the α-toxin skinned preparations. The cooperativity of the regulation of contraction varied among the differently skinned preparations between 3 (α-toxin) and 6 (triton X-100). The relationships between the calcium concentration and the isometric tension of the differently skinned preparations up to the optimal length for tension generation did not exhibit any length dependency. The length tension relationship, obtained from the maximal response at the highest calcium concentration is in line with that from other studies. The presence of intracellular proteins and membranes affects the regulation of contraction in smooth muscle of the femoral artery. Kluwer Academic Publishers, 2000 Van Heijst B. Department of Medical Physiology and Sports Medicine, Faculty of Medicine, Utrecht University, P.O. Box 80043, 3508 TA, Utrecht, The Netherlands aut Blangé T. Department of Physiology, Academic Medical Centre, University of Amsterdam, P.O. Box 22700, 1100 DE, Amsterdam, The Netherlands aut Jongsma H. Department of Medical Physiology and Sports Medicine, Faculty of Medicine, Utrecht University, P.O. Box 80043, 3508 TA, Utrecht, The Netherlands aut De Beer E. Department of Medical Physiology and Sports Medicine, Faculty of Medicine, Utrecht University, P.O. Box 80043, 3508 TA, Utrecht, The Netherlands aut Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/1(2000-01-01), 59-66 0142-4319 21:1<59 2000 21 10974 https://doi.org/10.1023/A:1005609319445 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1005609319445 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Van Heijst B. Department of Medical Physiology and Sports Medicine, Faculty of Medicine, Utrecht University, P.O. Box 80043, 3508 TA, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Blangé T. Department of Physiology, Academic Medical Centre, University of Amsterdam, P.O. Box 22700, 1100 DE, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Jongsma H. Department of Medical Physiology and Sports Medicine, Faculty of Medicine, Utrecht University, P.O. Box 80043, 3508 TA, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- De Beer E. Department of Medical Physiology and Sports Medicine, Faculty of Medicine, Utrecht University, P.O. Box 80043, 3508 TA, Utrecht, The Netherlands aut NATIONALLICENCE 773 0- Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/1(2000-01-01), 59-66 0142-4319 21:1<59 2000 21 10974 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer