caa a22 4500 475744748 CHVBK 20180406123510.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1023/A:1005682322132 doi (NATIONALLICENCE)springer-10.1023/A:1005682322132 Expression of cofilin isoforms during development of mouse striated muscles [Elektronische Daten] [Kurato Mohri, Hiromi Takano-Ohmuro, Kiyoko Nakashima, Kimihide Hayakawa, Takeshi Endo, Kazunori Hanaoka, Takashi Obinata] Cofilin (CF) is an actin regulatory protein that plays a critical role in actin filament dynamics in a variety of cells. Two cofilin isoforms, muscle-type (M-CF) and nonmuscle-type (NM-CF) encoded by different genes, exist in mammals; in the adult, the former is predominantly expressed in muscle tissues, while the latter is distributed in various non-muscle tissues (Ono et al., 1994). In this study, we examined cofilin isoform expression during skeletal and cardiac muscle development in mice using cDNA probes and antibodies which distinguish the isoforms. We found that the expression of M-CF was initiated in terminally differentiated myogenic cells in both the myotome and limb buds. In myogenic cell cultures, its expression occurred coupled with myotube formation. NM-CF was expressed in developing skeletal and cardiac muscles but disappeared from skeletal muscle during postnatal development, while its expression persisted in the heart, even in adult mice. A similar situation was observed in the heart of other mammals. Thus, it is likely that the both cofilin isoforms are involved in the regulation of actin assembly during myofibrillogenesis. Only M-CF could be involved in actin dynamics in mature skeletal muscle, while both isoforms could be in the mature heart. Kluwer Academic Publishers, 2000 Mohri Kurato Department of Biology, Faculty of Science, Chiba University, Yayoi-cho, Inage-ku, 263,-8522, Chiba, Japan aut Takano-Ohmuro Hiromi Department of Pharmacology, Faculty of Medicine, University of Tokyo, Hongo, Bunkyo-ku, 113-0033, Tokyo, Japan aut Nakashima Kiyoko Department of Biology, Faculty of Science, Chiba University, Yayoi-cho, Inage-ku, 263,-8522, Chiba, Japan aut Hayakawa Kimihide Department of Biology, Faculty of Science, Chiba University, Yayoi-cho, Inage-ku, 263,-8522, Chiba, Japan aut Endo Takeshi Department of Biology, Faculty of Science, Chiba University, Yayoi-cho, Inage-ku, 263,-8522, Chiba, Japan aut Hanaoka Kazunori Department of Bioscience, Kitasato University, Kitasato, 228-8555, Sagamihara, Japan aut Obinata Takashi Department of Biology, Faculty of Science, Chiba University, Yayoi-cho, Inage-ku, 263,-8522, Chiba, Japan aut Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/1(2000-01-01), 49-57 0142-4319 21:1<49 2000 21 10974 https://doi.org/10.1023/A:1005682322132 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1005682322132 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mohri Kurato Department of Biology, Faculty of Science, Chiba University, Yayoi-cho, Inage-ku, 263,-8522, Chiba, Japan aut NATIONALLICENCE 700 1- Takano-Ohmuro Hiromi Department of Pharmacology, Faculty of Medicine, University of Tokyo, Hongo, Bunkyo-ku, 113-0033, Tokyo, Japan aut NATIONALLICENCE 700 1- Nakashima Kiyoko Department of Biology, Faculty of Science, Chiba University, Yayoi-cho, Inage-ku, 263,-8522, Chiba, Japan aut NATIONALLICENCE 700 1- Hayakawa Kimihide Department of Biology, Faculty of Science, Chiba University, Yayoi-cho, Inage-ku, 263,-8522, Chiba, Japan aut NATIONALLICENCE 700 1- Endo Takeshi Department of Biology, Faculty of Science, Chiba University, Yayoi-cho, Inage-ku, 263,-8522, Chiba, Japan aut NATIONALLICENCE 700 1- Hanaoka Kazunori Department of Bioscience, Kitasato University, Kitasato, 228-8555, Sagamihara, Japan aut NATIONALLICENCE 700 1- Obinata Takashi Department of Biology, Faculty of Science, Chiba University, Yayoi-cho, Inage-ku, 263,-8522, Chiba, Japan aut NATIONALLICENCE 773 0- Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/1(2000-01-01), 49-57 0142-4319 21:1<49 2000 21 10974 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer