caa a22 4500 475744764 CHVBK 20180406123510.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1023/A:1005644931741 doi (NATIONALLICENCE)springer-10.1023/A:1005644931741 The spectrin skeleton of newly-invaginated plasma membrane [Elektronische Daten] [T. Herring, P. Juranka, J. McNally, H. Lesiuk, C. Morris] As a cell's shape and volume change, its surface area must re-adjust. How is the plasma membrane's spectrin skeleton implicated? For erythrocytes, cells of fixed surface area, spectrin responses to mechanical disturbances have been studied, but for more typical cells with changeable surface areas, they have not. In rapidly shrinking cells, surface membrane at an adherent substratum invaginates, forming transient vacuole-like dilations (VLDs). We exploited this readily inducible surface area perturbation to pose a simple question: is newly invaginated plasma membrane naked or is it supported by a spectrin skeleton? The spectrin skeleton was examined immunocytochemically in L6 cells (rat skeletal muscle) before and after VLD formation, using fixation in cold methanol and 4I12, an antibody against β-fodrin and β-spectrin. 4I12 was visualized by confocal fluorescence microscopy, while paired phase contrast images independently located the VLDs. To generate VLDs, cells were hypotonically swelled then reshrunk in isotonic medium. Swollen L6 cells maintained their plasma membrane (sarcolemma) spectrin skeleton. Within minutes of subsequent shrinkage, VLDs of 1-2 μm diameter invaginated at the substratum surface of myotubes. Both sarcolemma and VLDs were lined by a relatively uniform spectrin skeleton. Z-series suggested that some of the spectrin skeleton-lined sarcolemma became internalized as vacuoles. Kluwer Academic Publishers, 2000 Herring T. Loeb Health Research Institute, Ottawa Hospital, K1Y 4K9, Ottawa, Ontario, Canada aut Juranka P. Loeb Health Research Institute, Ottawa Hospital, K1Y 4K9, Ottawa, Ontario, Canada aut McNally J. Loeb Health Research Institute, Ottawa Hospital, K1Y 4K9, Ottawa, Ontario, Canada aut Lesiuk H. Loeb Health Research Institute, Ottawa Hospital, K1Y 4K9, Ottawa, Ontario, Canada aut Morris C. Loeb Health Research Institute, Ottawa Hospital, K1Y 4K9, Ottawa, Ontario, Canada aut Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/1(2000-01-01), 67-77 0142-4319 21:1<67 2000 21 10974 https://doi.org/10.1023/A:1005644931741 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1005644931741 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Herring T. Loeb Health Research Institute, Ottawa Hospital, K1Y 4K9, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- Juranka P. Loeb Health Research Institute, Ottawa Hospital, K1Y 4K9, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- McNally J. Loeb Health Research Institute, Ottawa Hospital, K1Y 4K9, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- Lesiuk H. Loeb Health Research Institute, Ottawa Hospital, K1Y 4K9, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- Morris C. Loeb Health Research Institute, Ottawa Hospital, K1Y 4K9, Ottawa, Ontario, Canada aut NATIONALLICENCE 773 0- Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/1(2000-01-01), 67-77 0142-4319 21:1<67 2000 21 10974 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer