caa a22 4500 475744977 CHVBK 20180406123510.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1023/A:1005689205862 doi (NATIONALLICENCE)springer-10.1023/A:1005689205862 Modifications of Ca2+ transport induced by glutathione in sarcoplasmic reticulum membranes of frog skeletal muscle [Elektronische Daten] [Silvia Belia, Jacopo Vecchiet, Leonardo Vecchiet, Giorgio Fanò] The Ca2+ transport across the membrane of vesicles purified from the sarcoplasmic reticulum (SR) of frog skeletal muscle is modified by raising the concentration of the reduced form of glutathione (GSH). Passive release of Ca2+ is inhibited through the direct action of GSH on ryanodine receptors while active uptake is increased by a dose-dependent stimulation of Ca2+ pumps (Ca2+-ATPase). These effects are physiological since the concentrations of GSH utilised (0.01-10.0 mM) are compatible with the in vivo concentration of this antioxidant. They are independent of the external Ca2+ concentration and are specific for the reduced form of glutathione, since the disulphide form (GSSG) or other GSH-derivatives do not induce these effects. Kluwer Academic Publishers, 2000 Belia Silvia Laboratorio Interuniversitario di Miologia, Università di Perugia and Università "G. d'Annunzio”, viadei Vestini 29, 66013, Chieti, Italy aut Vecchiet Jacopo Laboratorio Interuniversitario di Miologia, Università di Perugia and Università "G. d'Annunzio”, viadei Vestini 29, 66013, Chieti, Italy aut Vecchiet Leonardo Laboratorio Interuniversitario di Miologia, Università di Perugia and Università "G. d'Annunzio”, viadei Vestini 29, 66013, Chieti, Italy aut Fanò Giorgio Laboratorio Interuniversitario di Miologia, Università di Perugia and Università "G. d'Annunzio”, viadei Vestini 29, 66013, Chieti, Italy aut Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/3(2000-04-01), 279-283 0142-4319 21:3<279 2000 21 10974 https://doi.org/10.1023/A:1005689205862 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1005689205862 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Belia Silvia Laboratorio Interuniversitario di Miologia, Università di Perugia and Università "G. d'Annunzio”, viadei Vestini 29, 66013, Chieti, Italy aut NATIONALLICENCE 700 1- Vecchiet Jacopo Laboratorio Interuniversitario di Miologia, Università di Perugia and Università "G. d'Annunzio”, viadei Vestini 29, 66013, Chieti, Italy aut NATIONALLICENCE 700 1- Vecchiet Leonardo Laboratorio Interuniversitario di Miologia, Università di Perugia and Università "G. d'Annunzio”, viadei Vestini 29, 66013, Chieti, Italy aut NATIONALLICENCE 700 1- Fanò Giorgio Laboratorio Interuniversitario di Miologia, Università di Perugia and Università "G. d'Annunzio”, viadei Vestini 29, 66013, Chieti, Italy aut NATIONALLICENCE 773 0- Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/3(2000-04-01), 279-283 0142-4319 21:3<279 2000 21 10974 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer