caa a22 4500 475744985 CHVBK 20180406123510.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1023/A:1005672719974 doi (NATIONALLICENCE)springer-10.1023/A:1005672719974 Oxygenation and perfusion of rabbit tibialis anterior muscle subjected to different patterns of electrical stimulation [Elektronische Daten] [Adam Greenbaum, Jonathan Jarvis, Daniel O'Hare, Sanjiv Manek, Colin Green, John Pepper, C. Winlove, Stanley Salmons] Dual amperometric microelectrodes were used to measure local pO2 and perfusion at multiple sites in the fast-twitch tibialis anterior muscles of anaesthetized rabbits. Six muscles were stimulated continuously at 10, 5, or 2.5 Hz. For all three frequencies, perfusion declined to about 50% of resting levels and recovered after stimulation. These changes corresponded to a rise followed by a fall in extracellular pO2. The highest levels of pO2 were reached during stimulation at 10 Hz. Eight muscles were stimulated tetanically at 100 Hz for 200 ms with duty cycles that were varied between 1.3 and 20.0%. Perfusion rose to 8.7 ± 2.0 ml s−1 100 g−1 at a duty cycle of 5% and declined with further increases in duty cycle. pO2 was depressed for duty cycles less than 10% but rose above resting levels at higher duty cycles. It is suggested that the paradoxical combination of elevated pO2 and depressed perfusion is attributable to stimulation conditions that exceed the oxygen transport capacity of a fast muscle. Kluwer Academic Publishers, 2000 Greenbaum Adam Department of Anaesthesia and Critical Care, The Royal Brompton National Heart and Lung Hospital, SW3 6NP, London, UK aut Jarvis Jonathan Department of Human Anatomy and Cell Biology, The University of Liverpool, L69 3GE, Liverpool, UK aut O'Hare Daniel Department of Pharmacy, University of Brighton, BN2 4GJ, Brighton, UK aut Manek Sanjiv Department of Surgical Research, The Northwick Park Institute for Medical Research, HA1 3UJ, Middlesex, UK aut Green Colin Department of Surgical Research, The Northwick Park Institute for Medical Research, HA1 3UJ, Middlesex, UK aut Pepper John Department of Cardiothoracic Surgery, The Royal Brompton National Heart and Lung Hospital, SW3 6NP, London, UK aut Winlove C. The Physiological Flow Studies Group, Imperial College of Science, Technology & Medicine, SW7 2AZ, London, UK aut Salmons Stanley Department of Human Anatomy and Cell Biology, The University of Liverpool, L69 3GE, Liverpool, UK aut Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/3(2000-04-01), 285-291 0142-4319 21:3<285 2000 21 10974 https://doi.org/10.1023/A:1005672719974 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1005672719974 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Greenbaum Adam Department of Anaesthesia and Critical Care, The Royal Brompton National Heart and Lung Hospital, SW3 6NP, London, UK aut NATIONALLICENCE 700 1- Jarvis Jonathan Department of Human Anatomy and Cell Biology, The University of Liverpool, L69 3GE, Liverpool, UK aut NATIONALLICENCE 700 1- O'Hare Daniel Department of Pharmacy, University of Brighton, BN2 4GJ, Brighton, UK aut NATIONALLICENCE 700 1- Manek Sanjiv Department of Surgical Research, The Northwick Park Institute for Medical Research, HA1 3UJ, Middlesex, UK aut NATIONALLICENCE 700 1- Green Colin Department of Surgical Research, The Northwick Park Institute for Medical Research, HA1 3UJ, Middlesex, UK aut NATIONALLICENCE 700 1- Pepper John Department of Cardiothoracic Surgery, The Royal Brompton National Heart and Lung Hospital, SW3 6NP, London, UK aut NATIONALLICENCE 700 1- Winlove C. The Physiological Flow Studies Group, Imperial College of Science, Technology & Medicine, SW7 2AZ, London, UK aut NATIONALLICENCE 700 1- Salmons Stanley Department of Human Anatomy and Cell Biology, The University of Liverpool, L69 3GE, Liverpool, UK aut NATIONALLICENCE 773 0- Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/3(2000-04-01), 285-291 0142-4319 21:3<285 2000 21 10974 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer