caa a22 4500 475745000 CHVBK 20180406123510.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1023/A:1005680921792 doi (NATIONALLICENCE)springer-10.1023/A:1005680921792 Lack of coordinated changes in metabolic enzymes and myosin heavy chain isoforms in regenerated muscles of trained rats [Elektronische Daten] [A. Bigard, Ph. Mateo, H. Sanchez, B. Serrurier, R. Ventura-Clapier] We investigated training-induced changes in biochemical properties and myosin heavy chain (MHC) composition of regenerated (cardiotoxin-injected) plantaris muscles (PLA) in rats either maintained sedentary (S, n = 9) or endurance trained on a treadmill over a 8-week period (T, n = 7). Both endurance training and regeneration altered the pattern of fast MHC expression. An analysis of the two-way interaction between training and regeneration showed that the relative content of type IIa MHC was affected (P < 0.05). The 140% increase in type IIa MHC observed in regenerated PLA from T rats compared with nontreated muscle of S rats, exceeded the 102% increase resulting from the combination of regeneration alone (26%) and training alone (61%). A similar interaction between training and regeneration was shown for the percentage of fibres expressing either type IIa or type IIb MHC (P < 0.05). In contrast, a significant increase in the citrate synthase (CS) activity was shown in PLA as a result of endurance training, without specific effect of regeneration. Furthermore, training-induced changes in CK and LDH isoenzyme distribution occurred to a similar extent in regenerated and non-treated PLA muscles, and thus did not follow the changes in MHC isoforms. An increase in the mitochondrial CK isozyme activity (mi-CK) was shown in both non-treated and previously degenerated PLA muscles (123 and 117%, P < 0.01, respectively), without specific effect of regeneration. The ratio of mi-CK to CS activity, an estimate of the mitochondrial specific activity of mi-CK was significantly increased by training (P < 0.02) and decreased by regeneration (P < 0.05). Taken together, these data suggest that while training and regeneration have cumulative effects on the pattern of fast MHC expression, the training-induced changes in the energy metabolism shown in mature non-treated myofibres are similar to those observed in regenerated fibres. Kluwer Academic Publishers, 2000 Bigard A. Unité de Bioénergétique et Environnement, Centre de Recherches du Service de Santé des Armées, BP 87, 38702, La Tronche, France aut Mateo Ph Cardiologie Cellulaire et Moléculaire, U-446 INSERM, Faculté de Pharmacie, Université Paris-Sud, Chatenay-Malabry, France aut Sanchez H. Unité de Bioénergétique et Environnement, Centre de Recherches du Service de Santé des Armées, BP 87, 38702, La Tronche, France aut Serrurier B. Unité de Bioénergétique et Environnement, Centre de Recherches du Service de Santé des Armées, BP 87, 38702, La Tronche, France aut Ventura-Clapier R. Cardiologie Cellulaire et Moléculaire, U-446 INSERM, Faculté de Pharmacie, Université Paris-Sud, Chatenay-Malabry, France aut Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/3(2000-04-01), 269-278 0142-4319 21:3<269 2000 21 10974 https://doi.org/10.1023/A:1005680921792 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1005680921792 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bigard A. Unité de Bioénergétique et Environnement, Centre de Recherches du Service de Santé des Armées, BP 87, 38702, La Tronche, France aut NATIONALLICENCE 700 1- Mateo Ph Cardiologie Cellulaire et Moléculaire, U-446 INSERM, Faculté de Pharmacie, Université Paris-Sud, Chatenay-Malabry, France aut NATIONALLICENCE 700 1- Sanchez H. Unité de Bioénergétique et Environnement, Centre de Recherches du Service de Santé des Armées, BP 87, 38702, La Tronche, France aut NATIONALLICENCE 700 1- Serrurier B. Unité de Bioénergétique et Environnement, Centre de Recherches du Service de Santé des Armées, BP 87, 38702, La Tronche, France aut NATIONALLICENCE 700 1- Ventura-Clapier R. Cardiologie Cellulaire et Moléculaire, U-446 INSERM, Faculté de Pharmacie, Université Paris-Sud, Chatenay-Malabry, France aut NATIONALLICENCE 773 0- Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/3(2000-04-01), 269-278 0142-4319 21:3<269 2000 21 10974 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer