caa a22 4500 475745027 CHVBK 20180406123510.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1023/A:1005600118157 doi (NATIONALLICENCE)springer-10.1023/A:1005600118157 Influence of microtubules on vascular smooth muscle contraction [Elektronische Daten] [Dahua Zhang, Najia Jin, Rodney Rhoades, Kyle Yancey, Darl Swartz] Microtubules are ubiquitous in eukaryotic cells and play key roles in many cellular activities. The purpose of this study was to investigate the influence of microtubules on vascular smooth muscle contraction. Quantitative immunocytochemical analysis of rat aortic tissue revealed that, relative to the control group, colchicine (15 μM, 90 min) and nocodazole (15 μM, 90 min) decreased the microtubule density by 40-50% while taxol (10 μM, 90 min) increased the microtubule density by 33%. Isometric contraction studies demonstrated that both colchicine and nocodazole caused an upward shift in the phenylephrine (10−8 to 10−5 M) dose-response curve while taxol caused no significant change when compared to the control group. Potassium chloride (30 mM) induced 55 ± 5% P 0 contraction in DMSO treated vessel rings. The active tension increased to 73 ± 5% P 0 and 71 ± 6% P 0 after pretreatment of the aortic rings with colchicine or nocodazole, respectively. Taxol did not cause a significant change in the active tension (56 ± 7% P 0). These results indicate that microtubule depolymerization enhances isometric contraction of vascular smooth muscle and this enhanced contraction is not receptor dependent. Pretreatment of the aortic rings with an inhibitor of nitric oxide synthase (NOS) (N ω-nitro-L-arginine) did not change the increased contractile response to phenylephrine due to microtubule depolymerization suggesting that this phenomenon is not mediated by endothelium dependent relaxation. Kluwer Academic Publishers, 2000 Zhang Dahua Department of Anatomy and Cell Biology, Indiana University School of Medicine, 46202, Indianapolis, IN, USA aut Jin Najia Indiana University School of Medicine, 46202, Indianapolis, IN, USA aut Rhoades Rodney Indiana University School of Medicine, 46202, Indianapolis, IN, USA aut Yancey Kyle Department of Anatomy and Cell Biology, Indiana University School of Medicine, 46202, Indianapolis, IN, USA aut Swartz Darl Department of Anatomy and Cell Biology, Indiana University School of Medicine, 46202, Indianapolis, IN, USA aut Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/3(2000-04-01), 293-300 0142-4319 21:3<293 2000 21 10974 https://doi.org/10.1023/A:1005600118157 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1005600118157 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Dahua Department of Anatomy and Cell Biology, Indiana University School of Medicine, 46202, Indianapolis, IN, USA aut NATIONALLICENCE 700 1- Jin Najia Indiana University School of Medicine, 46202, Indianapolis, IN, USA aut NATIONALLICENCE 700 1- Rhoades Rodney Indiana University School of Medicine, 46202, Indianapolis, IN, USA aut NATIONALLICENCE 700 1- Yancey Kyle Department of Anatomy and Cell Biology, Indiana University School of Medicine, 46202, Indianapolis, IN, USA aut NATIONALLICENCE 700 1- Swartz Darl Department of Anatomy and Cell Biology, Indiana University School of Medicine, 46202, Indianapolis, IN, USA aut NATIONALLICENCE 773 0- Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/3(2000-04-01), 293-300 0142-4319 21:3<293 2000 21 10974 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer