caa a22 4500 475745035 CHVBK 20180406123510.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1023/A:1005670507906 doi (NATIONALLICENCE)springer-10.1023/A:1005670507906 Gelatinase-B (Matrix Metalloproteinase-9; MMP-9) secretion is involved in the migratory phase of human and murine muscle cell cultures [Elektronische Daten] [M. Lewis, H. Tippett, A. Sinanan, M. Morgan, N. Hunt] The remodelling of connective tissue components is a fundamental requirement for a number of pivotal processes in cell biology. These may include myoblast migration and fusion during development and regeneration. In other systems, similar biological processes are facilitated by secretion of the matrix metalloproteinases (MMPs), especially the gelatinases. This study investigated the activity of the gelatinases MMP-2 and 9 by zymography on cell conditioned media in cultures of cells derived from explants of the human masseter muscle and in the murine myoblast cell-line C2C12. Expression of MMP-9 by western blotting and TIMP-1, the major inhibitor of MMPs, by northern blotting, during all phases of myoblast proliferation, migration, alignment and fusion, was also measured. Irrespective of the origin of the cultures, MMP-9 activity was secreted only by single cell and pre-fusion cultures whilst MMP-2 activity was secreted at all stages as well as by myotubes. The loss of MMP-9 activity was due to the loss of MMP-9 protein expression. TIMP-1 mRNA was not detectable at the single cell stage but its expression increased as cells progressed through the pre-fusion and post-fusion stages to reach a maximal in myotube containing cultures. Migration of cells derived from human masseter muscle was inhibited, using a specific anti-MMP-9 blocking monoclonal antibody (6-6B). These data are consistent with the concept that regulation of matrix turnover via MMP-9 may be involved in the events leading to myotube formation, including migration. Loss of expression of this enzyme and expression of TIMP-1 mRNA is associated with myotube containing cultures. Consequently, the ratio between MMPs and TIMPs maybe important in determining myoblast migration and differentiation. Kluwer Academic Publishers, 2000 Lewis M. Department of Orthodontics, Division of Oral Biology and Pathology, Eastman Dental Institute for Oral Healthcare Sciences, University College London, 256 Gray's Inn Road, WC1X 8LD, London, UK aut Tippett H. Department of Orthodontics, Division of Oral Biology and Pathology, Eastman Dental Institute for Oral Healthcare Sciences, University College London, 256 Gray's Inn Road, WC1X 8LD, London, UK aut Sinanan A. Department of Orthodontics, Division of Oral Biology and Pathology, Eastman Dental Institute for Oral Healthcare Sciences, University College London, 256 Gray's Inn Road, WC1X 8LD, London, UK aut Morgan M. Department of Orthodontics, Division of Oral Biology and Pathology, Eastman Dental Institute for Oral Healthcare Sciences, University College London, 256 Gray's Inn Road, WC1X 8LD, London, UK aut Hunt N. Department of Orthodontics, Division of Oral Biology and Pathology, Eastman Dental Institute for Oral Healthcare Sciences, University College London, 256 Gray's Inn Road, WC1X 8LD, London, UK aut Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/3(2000-04-01), 223-233 0142-4319 21:3<223 2000 21 10974 https://doi.org/10.1023/A:1005670507906 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1005670507906 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lewis M. Department of Orthodontics, Division of Oral Biology and Pathology, Eastman Dental Institute for Oral Healthcare Sciences, University College London, 256 Gray's Inn Road, WC1X 8LD, London, UK aut NATIONALLICENCE 700 1- Tippett H. Department of Orthodontics, Division of Oral Biology and Pathology, Eastman Dental Institute for Oral Healthcare Sciences, University College London, 256 Gray's Inn Road, WC1X 8LD, London, UK aut NATIONALLICENCE 700 1- Sinanan A. Department of Orthodontics, Division of Oral Biology and Pathology, Eastman Dental Institute for Oral Healthcare Sciences, University College London, 256 Gray's Inn Road, WC1X 8LD, London, UK aut NATIONALLICENCE 700 1- Morgan M. Department of Orthodontics, Division of Oral Biology and Pathology, Eastman Dental Institute for Oral Healthcare Sciences, University College London, 256 Gray's Inn Road, WC1X 8LD, London, UK aut NATIONALLICENCE 700 1- Hunt N. Department of Orthodontics, Division of Oral Biology and Pathology, Eastman Dental Institute for Oral Healthcare Sciences, University College London, 256 Gray's Inn Road, WC1X 8LD, London, UK aut NATIONALLICENCE 773 0- Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/3(2000-04-01), 223-233 0142-4319 21:3<223 2000 21 10974 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer