caa a22 4500 475745132 CHVBK 20180406123511.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1023/A:1005650706464 doi (NATIONALLICENCE)springer-10.1023/A:1005650706464 Binding of filamin isoforms to myofibrils [Elektronische Daten] [Wen Chiang, Marion Greaser] Two filamin isoforms were purified from bovine tissues and characterized. Muscle filamin and nonmuscle filamin had different SDS gel mobilities, proteolytic digestion patterns, myofibrillar binding distributions and myofibril binding affinities. The muscle specific filamin had an apparent molecular weight of 265 kDa and bound primarily to the Z-lines of myofibrils but also to the I-bands near the Z-lines. The nonmuscle specific filamin had an apparent molecular weight of 275 kDa and bound exclusively to the Z-lines of myofibrils. The filamin-myofibril binding was studied quantitatively. Plotting bound fraction (mg filamin/mg myofibril) vs. equilibrium concentration of free filamin yielded a biphasic binding curve. The first hyperbolic binding phase described the binding of filamin to myofibrils but the second phase appeared to be nonspecific due to filamin aggregation. The muscle filamin had a significantly lower (P < 0.05) apparent binding affinity to myofibrils than nonmuscle filamin. However, the muscle filamin showed a significantly higher (P < 0.05) saturation value for myofibrils than nonmuscle filamin. The binding of phosphorylated filamin to myofibrils was significantly lower (P < 0.05) than the corresponding native proteins for both filamin isoforms. Kluwer Academic Publishers, 2000 Chiang Wen Muscle Biology Laboratory, University of Wisconsin-Madison, 53706, Madison, WI, USA aut Greaser Marion Muscle Biology Laboratory, University of Wisconsin-Madison, 53706, Madison, WI, USA aut Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/4(2000-05-01), 321-333 0142-4319 21:4<321 2000 21 10974 https://doi.org/10.1023/A:1005650706464 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1005650706464 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chiang Wen Muscle Biology Laboratory, University of Wisconsin-Madison, 53706, Madison, WI, USA aut NATIONALLICENCE 700 1- Greaser Marion Muscle Biology Laboratory, University of Wisconsin-Madison, 53706, Madison, WI, USA aut NATIONALLICENCE 773 0- Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/4(2000-05-01), 321-333 0142-4319 21:4<321 2000 21 10974 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer