caa a22 4500 475745205 CHVBK 20180406123511.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1023/A:1010362926221 doi (NATIONALLICENCE)springer-10.1023/A:1010362926221 Fibre types and Myosin heavy chain expression in the Ocular Medial Rectus Muscle of the Adult Rat [Elektronische Daten] [Branka Kranjc, Janez Sketelj, Anne Albis, Marko Ambrož, Ida Eržen] Myosin heavy chain (MHC) expression was determined immunohistochemically in individual muscle fibre types characterised by activities of ATPase and the key oxidative and glycolytic enzymes in rat ocular medial rectus (MR) muscles. In the global layer (GL), glycolytic activity of muscle fibres was higher and oxidative activity lower, than in the orbital layer (OL). Muscle fibres in the former displayed rosette-like organisation with a slow fibre surrounded by several fast fibres, which expressed either MHCIIa or MHCIIb, but many co-expressed both isoforms. In the OL some slow fibres co-expressed MHCIIa. Extraocular MHC isoform (MHCeom) could not be determined immunohistochemically and no pure MHCIIx/d containing fibres were found, suggesting that these isoforms, demonstrated electrophoretically, are co-expressed with others. Slow muscle fibres in both layers co-expressed MHCβ slow, MHCα cardiac and MHC-slow tonic. Neonatal isoform (MHCneo) was co-expressed in several fast and slow muscle fibres in the orbital, but not global layer. Slow fibres in the GL displayed very low oxidative activity. Electrophoretic analysis of ocular MR muscle homogenates revealed that about 50% of total MHC was MHCIIb, MHCeom was quite prominent (25%), and MHCIIa, MHCIIx/d and MHCI contributed each about 8%. MHCneo, MHCslow tonic and MHCα cardiac could not be identified as separate bands. Kluwer Academic Publishers, 2000 Kranjc Branka University Eye Clinic, Ljubljana, Slovenia aut Sketelj Janez Medical Faculty, Institute of Pathophysiology, Ljubljana, Slovenia aut Albis Anne Laboratoire de Biologie Physicochimique, Université Paris-Sud, Orsay, France aut Ambrož Marko Government Centre for Informatics, Ljubljana, Slovenia aut Eržen Ida Medical Faculty, Institute of Anatomy, Ljubljana, Slovenia aut Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/8(2000-11-01), 753-761 0142-4319 21:8<753 2000 21 10974 https://doi.org/10.1023/A:1010362926221 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1010362926221 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kranjc Branka University Eye Clinic, Ljubljana, Slovenia aut NATIONALLICENCE 700 1- Sketelj Janez Medical Faculty, Institute of Pathophysiology, Ljubljana, Slovenia aut NATIONALLICENCE 700 1- Albis Anne Laboratoire de Biologie Physicochimique, Université Paris-Sud, Orsay, France aut NATIONALLICENCE 700 1- Ambrož Marko Government Centre for Informatics, Ljubljana, Slovenia aut NATIONALLICENCE 700 1- Eržen Ida Medical Faculty, Institute of Anatomy, Ljubljana, Slovenia aut NATIONALLICENCE 773 0- Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/8(2000-11-01), 753-761 0142-4319 21:8<753 2000 21 10974 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer