caa a22 4500 475745302 CHVBK 20180406123511.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1023/A:1005657607591 doi (NATIONALLICENCE)springer-10.1023/A:1005657607591 Analysis for transcript expression of meltrin α in normal, regenerating, and denervated rat muscle [Elektronische Daten] [Antje Bornemann, Roland Kuschel, Atsuko Fujisawa-Sehara] Meltrin α (a disintegrin and metalloprotease (ADAM) 12) is a recently discovered molecule of the metalloprotease-disintegrin family which has been shown to participate in myotube formation in vitro and in myogenesis in vivo. In this study we investigated meltrin α in regenerating rat muscle, which is a condition where satellite cells (SC) contribute to myofiber growth by fusing with one another and with myotubes or muscle fibers. We studied meltrin α mRNA expression by RT-PCR and in situ-hybridization in normal adult muscle, in soleus muscle regenerating for 2, 5, or 10 days, and in muscle which had been denervated 1 week, 4 weeks, or 6 months previously. SC do not fuse after denervation. They detach from the principal muscle fiber. Immunohistochemistry using an antibody against M-cadherin was performed in parallel in order to identify SC. Messenger RNA as revealed by RT-PCR was absent in normal adult muscle, but present in regenerating and also in denervated muscle. Meltrin α transcript detected by in situ-hybridization was present in regenerating muscle only, not in normal or denervated muscle. It was localized to SC. Taken together, meltrin α is absent in normal muscle, and localized to SC in fusing conditions. After denervation, the transcript is upregulated. However, it is so lowly abundant that it fails to be detected by in situ-hybridization. This expression profile suggests a role for meltrin α in the fusion of SC with myotubes or muscle fibers, but not in SC adhesion to the adjacent myofiber in normal adult muscle. Kluwer Academic Publishers, 2000 Bornemann Antje Institute of Brain Research, University of Tübingen, Germany aut Kuschel Roland Institute of Brain Research, University of Tübingen, Germany aut Fujisawa-Sehara Atsuko Department of Cell Biology, The Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan aut Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/5(2000-07-01), 475-480 0142-4319 21:5<475 2000 21 10974 https://doi.org/10.1023/A:1005657607591 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1005657607591 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bornemann Antje Institute of Brain Research, University of Tübingen, Germany aut NATIONALLICENCE 700 1- Kuschel Roland Institute of Brain Research, University of Tübingen, Germany aut NATIONALLICENCE 700 1- Fujisawa-Sehara Atsuko Department of Cell Biology, The Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan aut NATIONALLICENCE 773 0- Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/5(2000-07-01), 475-480 0142-4319 21:5<475 2000 21 10974 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer