caa a22 4500 475745361 CHVBK 20180406123511.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1023/A:1005625302409 doi (NATIONALLICENCE)springer-10.1023/A:1005625302409 Chang K. Veterinary Molecular Medicine Laboratory, Department of Veterinary Pathology, University of Glasgow, Bearsden Road, G61 1QH aut Critical regulatory domains in intron 2 of a porcine sarcomeric myosin heavy chain gene [Elektronische Daten] [K. Chang] The porcine sarcomeric fast 2a myosin heavy chain (MyHC) gene was previously found to require a region extending 3′ from the transcriptional start site for high levels of expression. Here we established the existence of two novel opposing regulatory domains in intron 2. A positive regulatory element, defined to a 75bp region, resembles a TATA-less intronic promoter, with a consensus transcription initiation element. It can up-regulate its endogenous or a heterologous muscle promoter in a position specific manner, and on its own drive a reporter gene. In tandem with it is a dominant negative regulatory element, localised to a 81bp region, which can down-regulate its native gene and a heterologous muscle promoter. Bandshift and DNase I footprinting assays demonstrated that specific nuclear factors bound to both regulatory elements are distinctly different. Both elements appear to have no counterpart in intron 2 of the porcine fast 2x and 2b MyHC genes. Taken together, we demonstrate for the first time that a 5′-end terminal intron of a sarcomeric MyHC gene contains two critical regulatory domains, which may be involved in the complexity of temporo-spatial expression. Kluwer Academic Publishers, 2000 Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/5(2000-07-01), 451-461 0142-4319 21:5<451 2000 21 10974 https://doi.org/10.1023/A:1005625302409 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1005625302409 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Chang K. Veterinary Molecular Medicine Laboratory, Department of Veterinary Pathology, University of Glasgow, Bearsden Road, G61 1QH aut NATIONALLICENCE 773 0- Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/5(2000-07-01), 451-461 0142-4319 21:5<451 2000 21 10974 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer