caa a22 4500 475745418 CHVBK 20180406123511.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1023/A:1005660718348 doi (NATIONALLICENCE)springer-10.1023/A:1005660718348 The 5′-end of the porcine perinatal myosin heavy chain gene shows alternative splicing and is clustered with repeat elements [Elektronische Daten] [Nuno Costa, Nicolas Beuzen, Irene Johnston, Christine McGillivray, Yuh-man Sun, Kin Chang] The porcine perinatal myosin heavy chain (MyHC) is a major isoform in foetal skeletal muscles. We report here on its cDNA and genomic isolation, molecular characterisation and expression. Exon 2 and the first 4 bases of exon 3 of the perinatal MyHC gene, both part of the 5′-end untranslated region, showed differential splicing. About 2% of all perinatal MyHC transcripts of a 50-day-old foetus were without exon 2 and about half were without the 4 bases at the 5′-end of exon 3. Perinatal MyHC mRNA was expressed in all hind limb muscles of a 45-day-old foetus along with the slow and embryonic MyHC isoforms in the same fibres. Unlike other sarcomeric MyHCs reported to date, the porcine perinatal promoter is clustered with repeat elements (4 SINEs and 1 microsatellite) and is without a consensus TATA box at the predicted site upstream of exon 1. Nonetheless, in reporter gene transfections, its promoter was found to be highly muscle-specific. The absence of a TATA box may point to a fundamental difference in the regulatory function between the perinatal and adult MyHC isoforms. Kluwer Academic Publishers, 2000 Costa Nuno Veterinary Molecular Medicine Laboratory, Department of Veterinary Pathology, University of Glasgow, Bearsden Road, G61 1QH, UK aut Beuzen Nicolas Veterinary Molecular Medicine Laboratory, Department of Veterinary Pathology, University of Glasgow, Bearsden Road, G61 1QH, UK aut Johnston Irene Veterinary Molecular Medicine Laboratory, Department of Veterinary Pathology, University of Glasgow, Bearsden Road, G61 1QH, UK aut McGillivray Christine Veterinary Molecular Medicine Laboratory, Department of Veterinary Pathology, University of Glasgow, Bearsden Road, G61 1QH, UK aut Sun Yuh-man Veterinary Molecular Medicine Laboratory, Department of Veterinary Pathology, University of Glasgow, Bearsden Road, G61 1QH, UK aut Chang Kin Veterinary Molecular Medicine Laboratory, Department of Veterinary Pathology, University of Glasgow, Bearsden Road, G61 1QH, UK aut Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/2(2000-02-01), 183-188 0142-4319 21:2<183 2000 21 10974 https://doi.org/10.1023/A:1005660718348 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1005660718348 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Costa Nuno Veterinary Molecular Medicine Laboratory, Department of Veterinary Pathology, University of Glasgow, Bearsden Road, G61 1QH, UK aut NATIONALLICENCE 700 1- Beuzen Nicolas Veterinary Molecular Medicine Laboratory, Department of Veterinary Pathology, University of Glasgow, Bearsden Road, G61 1QH, UK aut NATIONALLICENCE 700 1- Johnston Irene Veterinary Molecular Medicine Laboratory, Department of Veterinary Pathology, University of Glasgow, Bearsden Road, G61 1QH, UK aut NATIONALLICENCE 700 1- McGillivray Christine Veterinary Molecular Medicine Laboratory, Department of Veterinary Pathology, University of Glasgow, Bearsden Road, G61 1QH, UK aut NATIONALLICENCE 700 1- Sun Yuh-man Veterinary Molecular Medicine Laboratory, Department of Veterinary Pathology, University of Glasgow, Bearsden Road, G61 1QH, UK aut NATIONALLICENCE 700 1- Chang Kin Veterinary Molecular Medicine Laboratory, Department of Veterinary Pathology, University of Glasgow, Bearsden Road, G61 1QH, UK aut NATIONALLICENCE 773 0- Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/2(2000-02-01), 183-188 0142-4319 21:2<183 2000 21 10974 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer