caa a22 4500 475745434 CHVBK 20180406123511.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1023/A:1005639229497 doi (NATIONALLICENCE)springer-10.1023/A:1005639229497 Evidence for differential post-translational modifications of slow myosin heavy chain during murine skeletal muscle development [Elektronische Daten] [A. Maggs, P. Taylor-Harris, M. Peckham, S. Hughes] The contractile properties of muscle fibres are, in part, determined by the myosin heavy chain (MyHC) isoforms they express. Using monoclonal antibodies, we show that at least three forms of slow twitch MyHC accumulate sequentially during mouse fetal development and that slow MyHC maturation in slow fibres occurs before expression of the adult fast MyHCs in fast fibres. Expression of deletion derivatives of β-cardiac MyHC cDNA shows that the slow MyHC epitopes that are detected in adult but not in young animals are located near the N-terminus. The same N-terminal region of various fast MyHC molecules contains a conserved epitope that can, on occasions, be observed when slow MyHC cDNA is expressed in non-muscle cells. The results raise the possibility that the N-terminal epitopes result from post-translational modification of the MyHC and that a sequence of slow and fast MyHC isoform post-translational modifications plays a significant role during development of murine muscle fibres. Kluwer Academic Publishers, 2000 Maggs A. MRC Muscle and Cell Motility Unit and Developmental Biology Research Centre, The Randall Institute, King's College London, WC2B 5RL, London, UK aut Taylor-Harris P. MRC Muscle and Cell Motility Unit and Developmental Biology Research Centre, The Randall Institute, King's College London, WC2B 5RL, London, UK aut Peckham M. MRC Muscle and Cell Motility Unit and Developmental Biology Research Centre, The Randall Institute, King's College London, WC2B 5RL, London, UK aut Hughes S. MRC Muscle and Cell Motility Unit and Developmental Biology Research Centre, The Randall Institute, King's College London, WC2B 5RL, London, UK aut Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/2(2000-02-01), 101-113 0142-4319 21:2<101 2000 21 10974 https://doi.org/10.1023/A:1005639229497 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1005639229497 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Maggs A. MRC Muscle and Cell Motility Unit and Developmental Biology Research Centre, The Randall Institute, King's College London, WC2B 5RL, London, UK aut NATIONALLICENCE 700 1- Taylor-Harris P. MRC Muscle and Cell Motility Unit and Developmental Biology Research Centre, The Randall Institute, King's College London, WC2B 5RL, London, UK aut NATIONALLICENCE 700 1- Peckham M. MRC Muscle and Cell Motility Unit and Developmental Biology Research Centre, The Randall Institute, King's College London, WC2B 5RL, London, UK aut NATIONALLICENCE 700 1- Hughes S. MRC Muscle and Cell Motility Unit and Developmental Biology Research Centre, The Randall Institute, King's College London, WC2B 5RL, London, UK aut NATIONALLICENCE 773 0- Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/2(2000-02-01), 101-113 0142-4319 21:2<101 2000 21 10974 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer