caa a22 4500 475745450 CHVBK 20180406123512.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1023/A:1005671319604 doi (NATIONALLICENCE)springer-10.1023/A:1005671319604 S-NO-actin: S-nitrosylation kinetics and the effect on isolated vascular smooth muscle [Elektronische Daten] [Isabella Dalle-donne, Aldo Milzani/snm>, Daniela Giustarini, Paolo Simplicio, Roberto Colombo, Ranieri Rossi] We describe the modification of reactive actin sulfhydryls by S-nitrosoglutathione. Kinetics of S-nitrosylation and denitrosylation suggest that only one cysteine of actin is involved in the reactions. By using the bifunctional sulfhydryl cross-linking reagent N,N′-1,4-phenylenebismaleimide and the monofunctional reagent N-iodoacetyl-N′-(5-sulpho-1-naphthyl)ethylenediamine, we identified this residue as Cys374. The time course of filament formation followed by high-shear viscosity changes revealed that S-nitrosylated G-actin polymerizes less efficiently than native monomers. The observed decrease in specific viscosity at steady state is due mainly to a marked inhibition of filament end-to-end annealing and, partially, to a reduction in F-actin concentration. Finally, S-nitrosylated actin acts as nitric oxide donor showing a fast, potent vasodilating activity at unusually low concentrations, being comparable with that of low molecular weight nitrosothiols. Kluwer Academic Publishers, 2000 Dalle-donne Isabella Lab. Biochem. Biophys. Cytoskel., Department of Biology, University of Milan, via Celoria 26, I-20133, Milan, Italy aut Milzani/snm> Aldo Lab. Biochem. Biophys. Cytoskel., Department of Biology, University of Milan, via Celoria 26, I-20133, Milan, Italy aut Giustarini Daniela Pharmacology Section, Institute for Mental and Nervous Diseases, University of Siena, via A. Moro 4, I-53100, Siena, Italy aut Simplicio Paolo Pharmacology Section, Institute for Mental and Nervous Diseases, University of Siena, via A. Moro 4, I-53100, Siena, Italy aut Colombo Roberto Lab. Biochem. Biophys. Cytoskel., Department of Biology, University of Milan, via Celoria 26, I-20133, Milan, Italy aut Rossi Ranieri Pharmacology Section, Institute for Mental and Nervous Diseases, University of Siena, via A. Moro 4, I-53100, Siena, Italy aut Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/2(2000-02-01), 171-181 0142-4319 21:2<171 2000 21 10974 https://doi.org/10.1023/A:1005671319604 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/A:1005671319604 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dalle-donne Isabella Lab. Biochem. Biophys. Cytoskel., Department of Biology, University of Milan, via Celoria 26, I-20133, Milan, Italy aut NATIONALLICENCE 700 1- Milzani/snm> Aldo Lab. Biochem. Biophys. Cytoskel., Department of Biology, University of Milan, via Celoria 26, I-20133, Milan, Italy aut NATIONALLICENCE 700 1- Giustarini Daniela Pharmacology Section, Institute for Mental and Nervous Diseases, University of Siena, via A. Moro 4, I-53100, Siena, Italy aut NATIONALLICENCE 700 1- Simplicio Paolo Pharmacology Section, Institute for Mental and Nervous Diseases, University of Siena, via A. Moro 4, I-53100, Siena, Italy aut NATIONALLICENCE 700 1- Colombo Roberto Lab. Biochem. Biophys. Cytoskel., Department of Biology, University of Milan, via Celoria 26, I-20133, Milan, Italy aut NATIONALLICENCE 700 1- Rossi Ranieri Pharmacology Section, Institute for Mental and Nervous Diseases, University of Siena, via A. Moro 4, I-53100, Siena, Italy aut NATIONALLICENCE 773 0- Journal of Muscle Research & Cell Motility Kluwer Academic Publishers 21/2(2000-02-01), 171-181 0142-4319 21:2<171 2000 21 10974 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer